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SK-PC-B70M confers anti-oxidant activity and reduces Abeta levels in the brain of Tg2576 mice.

Abstract
SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Abeta(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Abeta(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66% and 79% of the control Tg2576 mice. Anti-Abeta antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H(2)O(2) in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice.
AuthorsJi-Seon Seo, Tae-Kyung Kim, Yea-Hyun Leem, Kang-Woo Lee, Sun-Kyu Park, In-Sun Baek, Kyoung-Shim Kim, Guang-Jin Im, Soo Min Lee, Yang Hae Park, Pyung-Lim Han
JournalBrain research (Brain Res) Vol. 1261 Pg. 100-8 (Mar 19 2009) ISSN: 1872-6240 [Electronic] Netherlands
PMID19401163 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Amyloid beta-Peptides
  • Amyloid beta-Protein Precursor
  • Antioxidants
  • Peptide Fragments
  • Reactive Oxygen Species
  • SK-PC-B70M
  • Saponins
  • Malondialdehyde
  • Hydrogen Peroxide
Topics
  • Amyloid beta-Peptides (metabolism)
  • Amyloid beta-Protein Precursor (genetics)
  • Analysis of Variance
  • Animals
  • Antioxidants (pharmacology)
  • Blotting, Western
  • Cell Death (drug effects)
  • Cells, Cultured
  • Cerebral Cortex (cytology, drug effects, metabolism)
  • Cytoprotection
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Fluorescent Antibody Technique
  • Hydrogen Peroxide (metabolism)
  • Male
  • Malondialdehyde (metabolism)
  • Mice
  • Mice, Transgenic
  • Neurons (drug effects, metabolism)
  • Peptide Fragments (metabolism)
  • Plaque, Amyloid (metabolism)
  • Reactive Oxygen Species (metabolism)
  • Saponins (pharmacology)

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