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Elevated levels of thymidine kinase 1 peptide in serum from patients with breast cancer.

AbstractOBJECTIVES:
Thymidine kinase (TK) has an important role in DNA synthesis and is thus related to cell proliferation and turn-over. Traditionally, TK has been measured by enzymatic activity or radioimmunoassays. These assays are difficult to adapt to random access instruments. The aim of this study was to evaluate a new immunological sandwich assay for detection of TK peptides in serum from breast cancer patients.
MATERIAL AND METHODS:
Serum samples were collected from patients with breast cancer and stored frozen at -70 degrees C. The samples were collected after surgery, after metastatic tumor recurrence and after chemotherapy due to tumour recurrence. Patients' serum samples were analysed by the TK enzyme-linked immunosorbent assay (ELISA).
RESULTS:
In receiver operating characteristics (ROC) analyses of TK1 for diagnosis of breast cancer, the area under the curve (AUC) collected four weeks after surgery was 0.56 (95% CI 0.47-0.65), for samples collected postsurgically after tumour recurrence 0.73 (95% CI 0.65-0.80), and after chemotherapy 0.64 (95% CI 0.56-0.72).
CONCLUSIONS:
This study indicates that the tumour proliferation marker TK has a potential as a serum marker in breast cancer. Further studies are warranted to verify this observation.
AuthorsLena Carlsson, Anders Larsson, Henrik Lindman
JournalUpsala journal of medical sciences (Ups J Med Sci) Vol. 114 Issue 2 Pg. 116-20 ( 2009) ISSN: 2000-1967 [Electronic] Sweden
PMID19396699 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Biomarkers, Tumor
  • Peptide Fragments
  • Thymidine Kinase
  • thymidine kinase 1
Topics
  • Biomarkers, Tumor (blood)
  • Breast Neoplasms (blood, enzymology)
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Peptide Fragments (blood)
  • ROC Curve
  • Sensitivity and Specificity
  • Thymidine Kinase (blood, chemistry)

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