Abstract | OBJECTIVES:
Thymidine kinase (TK) has an important role in DNA synthesis and is thus related to cell proliferation and turn-over. Traditionally, TK has been measured by enzymatic activity or radioimmunoassays. These assays are difficult to adapt to random access instruments. The aim of this study was to evaluate a new immunological sandwich assay for detection of TK peptides in serum from breast cancer patients. MATERIAL AND METHODS: Serum samples were collected from patients with breast cancer and stored frozen at -70 degrees C. The samples were collected after surgery, after metastatic tumor recurrence and after chemotherapy due to tumour recurrence. Patients' serum samples were analysed by the TK enzyme-linked immunosorbent assay (ELISA). RESULTS: In receiver operating characteristics (ROC) analyses of TK1 for diagnosis of breast cancer, the area under the curve (AUC) collected four weeks after surgery was 0.56 (95% CI 0.47-0.65), for samples collected postsurgically after tumour recurrence 0.73 (95% CI 0.65-0.80), and after chemotherapy 0.64 (95% CI 0.56-0.72). CONCLUSIONS: This study indicates that the tumour proliferation marker TK has a potential as a serum marker in breast cancer. Further studies are warranted to verify this observation.
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Authors | Lena Carlsson, Anders Larsson, Henrik Lindman |
Journal | Upsala journal of medical sciences
(Ups J Med Sci)
Vol. 114
Issue 2
Pg. 116-20
( 2009)
ISSN: 2000-1967 [Electronic] Sweden |
PMID | 19396699
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Peptide Fragments
- Thymidine Kinase
- thymidine kinase 1
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Topics |
- Biomarkers, Tumor
(blood)
- Breast Neoplasms
(blood, enzymology)
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Peptide Fragments
(blood)
- ROC Curve
- Sensitivity and Specificity
- Thymidine Kinase
(blood, chemistry)
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