Abstract |
Until now, serologic tests that distinguish the closely related human T-cell lymphotropic virus types I (HTLV-I) and II ( HTLV-II) infections have not been available. Synthetic peptide assays, employing peptides derived from the core and envelope proteins of HTLV-I and HTLV-II (SynthEIA and Select-HTLV tests), were evaluated for the ability to serologically discriminate HTLV-I and HTLV-II infections. Of 32 HTLV-I- and 57 HTLV-II-positive serum specimens from individuals whose infections were confirmed by polymerase chain reaction, the SynthEIA test categorized 29 (91%) as HTLV-I and 50 (88%) as HTLV-II, and 10 (11%) were nontypeable. In contrast, the Select-HTLV test categorized 32 (100%) as HTLV-I and 55 (96%) as HTLV-II, and 2 (2%) were nontypeable. The specificity of both the assays in seropositive serum specimens was 100% in that none of the specimens were incorrectly classified. Additional serum specimens obtained from clinically diseased patients from the United States (n = 8) and asymptomatic carriers and patients from Japan (an endemic population for HTLV-I; n = 40) were categorized as HTLV-I by at least one of the assays, while serum specimens from Guaymi Indians from Panama (an endemic population for HTLV-II; n = 13) were categorized as HTLV-II. Thus, peptide enzyme immunoassays appear to represent a simple technique employing chemically synthesized antigens for discrimination between antibodies of HTLV-I and HTLV-II.
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Authors | R B Lal, W Heneine, D L Rudolph, W B Present, D Hofhienz, T M Hartley, R F Khabbaz, J E Kaplan |
Journal | Journal of clinical microbiology
(J Clin Microbiol)
Vol. 29
Issue 10
Pg. 2253-8
(Oct 1991)
ISSN: 0095-1137 [Print] United States |
PMID | 1939580
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antibodies, Viral
- DNA, Viral
- Peptides
- Viral Proteins
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Topics |
- Antibodies, Viral
(blood)
- DNA, Viral
(genetics)
- Evaluation Studies as Topic
- Female
- HTLV-I Infections
(diagnosis, immunology)
- HTLV-II Infections
(diagnosis, immunology)
- Human T-lymphotropic virus 1
(genetics, immunology)
- Human T-lymphotropic virus 2
(genetics, immunology)
- Humans
- Immunoassay
(methods)
- Male
- Molecular Sequence Data
- Peptides
(immunology)
- Viral Proteins
(analysis, immunology)
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