Abstract | BACKGROUND: METHODS: Saliva/standard samples were C18-SPE extracted, dried and resuspended. E and F were analysed by isocratic RP-HPLC ( acetonitrile/water 27/73%) and UV detection. In the morning and in the evening Salivette stimulated saliva specimens were collected from healthy volunteers. RESULTS: The E and F calibration curve ranges were 11.0-110.0 and 5.5-55.0 nmol/l respectively. The LOD was 0.2 and 0.1 nmol/l for E and F respectively. The intra and inter assay CVs were respectively 2.7-6.6 and 5.6-7.0% for E and 5.8-7.0 and 11.7-13.1% for F. The E and F spiked saliva sample recovery was 99% and 88% respectively. Saliva specimen stability was validated. E and F saliva levels in healthy volunteers were significantly (p<0.001) higher at 8 a.m. compared with 11 p.m. (26.4+/-8.9 vs. 4.3+/-2.9 nmol/l for E; 11.1+/-4.0 vs. 2.5+/-1.5 nmol/l for F, respectively). CONCLUSIONS: This method is suitable for periodic analyses in a clinical biochemistry laboratory for endocrinology investigation purposes, simultaneously analysing E and F levels in a saliva specimen.
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Authors | Elio F De Palo, Giorgia Antonelli, Arianna Benetazzo, Maddalena Prearo, Rosalba Gatti |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 405
Issue 1-2
Pg. 60-5
(Jul 2009)
ISSN: 1873-3492 [Electronic] Netherlands |
PMID | 19393639
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Adult
- Chromatography, High Pressure Liquid
(methods)
- Cortisone
(analysis)
- Female
- Humans
- Hydrocortisone
(analysis)
- Hydrophobic and Hydrophilic Interactions
- Indicator Dilution Techniques
- Male
- Saliva
(chemistry)
- Solid Phase Extraction
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