Establishment of experimental eosinophilic vasculitis by IgE-mediated cutaneous reverse passive arthus reaction.

Abstract

Prominent eosinophil infiltration is a characteristic of some forms of vasculitis, such as Churg-Strauss syndrome, also known as allergic granulomatous vasculitis. In the current study, we established a mouse model of cutaneous eosinophilic vasculitis by the cutaneous reverse passive Arthus reaction using IgE injection instead of IgG. Wild-type C57BL/6 mice were injected with IgE anti-trinitrophenyl antibodies, followed immediately by intravenous administration of trinitrophenyl bovine serum albumin. IgE-mediated immune complex challenge induced substantial hemorrhage with marked infiltration of eosinophils in which neutrophils, mast cells, and macrophages were also mixed. This finding contrasted remarkably with the neutrophil-dominant infiltration pattern in IgG-mediated immune complex challenge. In the lesion, the expression level of monocyte chemotactic protein-3 was increased, and anti-monocyte chemotactic protein-3 treatment resulted in a significant but incomplete blockade of eosinophil recruitment. Furthermore, mice lacking E-selectin, P-selectin, L-selectin, or intercellular adhesion molecule-1, as well as wild-type mice that received anti-vascular cell adhesion molecule-1-blocking antibodies were assessed for the IgE-mediated Arthus reaction. After 24 hours, the loss of P-selectin resulted in a significant reduction in eosinophil accumulation compared with both wild-type mice and other mouse mutants. Collectively, the Fc class of immunoglobulins, which forms these immune complexes, critically determines the disease manifestation of vasculitis. The IgE-mediated cutaneous reverse passive Arthus reaction may serve as an experimental model for cutaneous eosinophilic infiltration in vasculitis as well as in other diseases.

• Authors: Takayuki Ishii, Tomoyuki Fujita, Takashi Matsushita, Koichi Yanaba, Minoru Hasegawa, Hiroko Nakashima, Fumihide Ogawa, Kazuhiro Shimizu, Kazuhiko Takehara, Thomas F Tedder, Shinichi Sato, Manabu Fujimoto
• Journal: The American journal of pathology (Am J Pathol) Vol. 174 Issue 6 Pg. 2225-33 (Jun 2009) ISSN: 1525-2191 [Electronic] United States
• PMID: 19389931 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigen-Antibody Complex
• E-Selectin
• Immunoglobulin G
• P-Selectin
• Intercellular Adhesion Molecule-1
• L-Selectin
• Immunoglobulin E

Topics

• Animals
• Antigen-Antibody Complex
• Arthus Reaction (immunology)
• Disease Models, Animal
• E-Selectin (immunology)
• Eosinophilia (immunology, pathology)
• Immunoglobulin E (immunology)
• Immunoglobulin G (immunology)
• Immunohistochemistry
• Intercellular Adhesion Molecule-1 (immunology)
• L-Selectin (immunology)
• Mice
• Mice, Inbred C57BL
• P-Selectin (immunology)
• Reverse Transcriptase Polymerase Chain Reaction
• Skin Diseases (immunology, pathology)
• Vasculitis, Leukocytoclastic, Cutaneous (immunology, pathology)