| Abstract | Previously, treatment with anti-gpTNFalpha antibody enhanced TNFalpha mRNA expression in pulmonary granulomas microdissected from non-vaccinated guinea pigs, and modified splenic granuloma architecture. In this study, pleural fluid, cells, and granulomatous tissues were collected 3, 5, and 8 days post-pleurisy induction in guinea pigs treated with anti-gpTNFalpha or normal serum control. Neutralizing TNFalpha reduced the percentage of macrophages in the pleural exudate while increasing the proportions of neutrophils and lymphocytes. Cell-associated mycobacterial loads were increased in guinea pigs treated with anti-gpTNFalpha antibody. Cells from the pleural exudate in both treatment groups at day 3 expressed predominantly TNFalpha and IFNgamma mRNA. By day 5, treatment with anti-gpTNFalpha antibody significantly reduced TNFalpha mRNA and increased TGFbeta and iNOS mRNA expression, a transition which did not occur in the control group until day 8. TNFalpha mRNA overwhelmed the cytokine milieu of microdissected pleural granulomas in the control group at day 3 whereas TNFalpha, IFNgamma, and TGFbeta mRNA dominated the anti-gpTNFalpha-treated group. At day 8, granulomas from the control group began shifting towards an anti-inflammatory profile with increased levels of TGFbeta mRNA. Neutralization of TNFalpha hastened the transition to an anti-inflammatory cytokine response in guinea pig pleural granulomas and exudate cells. |
| Authors | Lan H Ly, Amminikutty Jeevan, David N McMurray
(Affiliation: Department of Microbial and Molecular Pathogenesis, College of Medicine, Texas A&M Health Science Center, 463 Reynolds Medical Building, College Station, TX, USA. lhly at medicine.tamhsc.edu)
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| Journal | Microbes and infection / Institut Pasteur
(Microbes Infect)
2009 May-Jun
Vol. 11
Issue 6-7
Pg. 680-8
ISSN: 1769-714X [Electronic] France |
| PMID | 19389482
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
|
| Chemical References |
- Cytokines
- Tumor Necrosis Factor-alpha
|
| Topics |
- Animals
- Cytokines
(biosynthesis)
- Exudates and Transudates
(cytology)
- Granuloma
(immunology)
- Guinea Pigs
- Inflammation
(pathology)
- Macrophages
(immunology)
- Neutrophils
(immunology)
- T-Lymphocytes
(immunology)
- Tuberculosis, Pleural
(immunology, pathology)
- Tumor Necrosis Factor-alpha
(antagonists & inhibitors)
|
