| Abstract | The role of CD8 T cells in anti-tuberculosis immunity in humans remains unknown, and studies of CD8 T cell-mediated protection against tuberculosis in mice have yielded controversial results. Unlike mice, humans and nonhuman primates share a number of important features of the immune system that relate directly to the specificity and functions of CD8 T cells, such as the expression of group 1 CD1 proteins that are capable of presenting Mycobacterium tuberculosis lipids antigens and the cytotoxic/bactericidal protein granulysin. Employing a more relevant nonhuman primate model of human tuberculosis, we examined the contribution of BCG- or M. tuberculosis-elicited CD8 T cells to vaccine-induced immunity against tuberculosis. CD8 depletion compromised BCG vaccine-induced immune control of M. tuberculosis replication in the vaccinated rhesus macaques. Depletion of CD8 T cells in BCG-vaccinated rhesus macaques led to a significant decrease in the vaccine-induced immunity against tuberculosis. Consistently, depletion of CD8 T cells in rhesus macaques that had been previously infected with M. tuberculosis and cured by antibiotic therapy also resulted in a loss of anti-tuberculosis immunity upon M. tuberculosis re-infection. The current study demonstrates a major role for CD8 T cells in anti-tuberculosis immunity, and supports the view that CD8 T cells should be included in strategies for development of new tuberculosis vaccines and immunotherapeutics. |
| Authors | Crystal Y Chen, Dan Huang, Richard C Wang, Ling Shen, Gucheng Zeng, Shuyun Yao, Yun Shen, Lisa Halliday, Jeff Fortman, Milton McAllister, Jim Estep, Robert Hunt, Daphne Vasconcelos, George Du, Steven A Porcelli, Michelle H Larsen, William R Jacobs Jr, Barton F Haynes, Norman L Letvin, Zheng W Chen
(Affiliation: Department of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of Medicine, Chicago, Illinois, USA.)
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| Journal | PLoS pathogens
(PLoS Pathog)
Vol. 5
Issue 4
Pg. e1000392
(Apr 2009)
ISSN: 1553-7374 [Electronic] United States |
| PMID | 19381260
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Antigens, CD8
- BCG Vaccine
|
| Topics |
- Animals
- Antigens, CD8
(immunology)
- BCG Vaccine
(immunology)
- CD8-Positive T-Lymphocytes
(immunology)
- Disease Models, Animal
- Humans
- Macaca mulatta
- Mycobacterium tuberculosis
(immunology, physiology)
- Tuberculosis
(immunology, prevention & control)
- Vaccination
(veterinary)
|
