Abstract |
Leucine aminopeptidase A (LapA) is a late wound-response gene of tomato (Solanum lycopersicum). To elucidate the role of LapA, transgenic plants that overexpressed or abolished LapA gene expression were used. The early wound-response gene RNA levels were similar in wild-type and Lap-silenced (LapA-SI), -antisense (LapA-AS), and -overexpressing (LapA-OX) plants. By contrast, late wound-response gene RNA levels and protection against Manduca sexta damage were influenced by LapA RNA and protein levels. While LapA-OX plants had elevated levels of LapA RNAs and protein, ectopic expression of LapA was not sufficient to induce Pin (Ser proteinase inhibitor) or PPO ( polyphenol oxidase) transcripts in nonwounded leaves. M. sexta larvae damaged less foliage and displayed delays in growth and development when feeding on LapA-OX plants. By contrast, LapA-SI and LapA-AS lines had lower levels of Pin and PPO RNAs than wild-type controls. Furthermore, larvae consumed more foliage and attained larger masses when feeding on LapA-SI plants. Jasmonic acid (JA) did not complement the wound-signaling phenotype of LapA-SI plants. Based on root elongation in the presence of JA, JA perception appeared to be intact in LapA-SI lines. Collectively, these data suggested that LAP-A has a role in modulating essential defenses against herbivores by promoting late wound responses and acting downstream of JA biosynthesis and perception.
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Authors | Jonathan H Fowler, Javier Narváez-Vásquez, Dale N Aromdee, Véronique Pautot, Frances M Holzer, Linda L Walling |
Journal | The Plant cell
(Plant Cell)
Vol. 21
Issue 4
Pg. 1239-51
(Apr 2009)
ISSN: 1040-4651 [Print] England |
PMID | 19376935
(Publication Type: Journal Article)
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Chemical References |
- Cyclopentanes
- Oxylipins
- Plant Growth Regulators
- Plant Proteins
- jasmonic acid
- Leucyl Aminopeptidase
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Topics |
- Animals
- Cyclopentanes
(metabolism, pharmacology)
- Feeding Behavior
- Gene Silencing
- Leucyl Aminopeptidase
(metabolism, physiology)
- Solanum lycopersicum
(drug effects, enzymology, metabolism)
- Manduca
(growth & development, physiology)
- Oxylipins
(metabolism, pharmacology)
- Phenotype
- Plant Growth Regulators
(metabolism, pharmacology)
- Plant Proteins
(metabolism, physiology)
- Plants, Genetically Modified
(drug effects, metabolism)
- Signal Transduction
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