Abstract | AIM: METHODS: Six pigs were infused with 2.8 g of BDT for 150 min. Six control pigs were infused with albumin sham solution for 150 min. Bile samples were analysed for bile acid- and phospholipid-secretion rates. Bile and serum samples were analysed for bilirubin concentration. Liver biopsies were obtained for scanning electron microscopic studies (SEM). RESULTS: Biliary bile acid secretion fell by 7.7% and biliary phospholipid secretion rate fell by 88% after BDT infusion. Thus, infusion of BDT for 150 min did not cause intrahepatic cholestasis. SEM showed some variability in the size of canalicular membrane microvilli, but no evidence of gross destruction. CONCLUSION:
BDT overload markedly lowers biliary phospholipid secretion. In contrast to UCB, BDT does not induce canalicular membrane damage nor cause intrahepatic cholestasis. Sustained, marked inhibition of phospholipid secretion does therefore not adequately explain UCB-induced cholestasis. Accumulation of UCB in the canalicular membrane may be the important factor in the pathogenesis of canalicular membrane lesions and intrahepatic cholestasis during UCB overload.
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Authors | Knut J Labori, Torstein Lyberg, Morten G Raeder |
Journal | Injury
(Injury)
Vol. 40
Issue 8
Pg. 868-72
(Aug 2009)
ISSN: 1879-0267 [Electronic] Netherlands |
PMID | 19376516
(Publication Type: Journal Article)
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Chemical References |
- Taurine
- bilirubin ditaurine
- Bilirubin
|
Topics |
- Animals
- Bile
(metabolism)
- Bilirubin
(administration & dosage, analogs & derivatives)
- Cholestasis, Intrahepatic
(etiology)
- Liver
(metabolism, ultrastructure)
- Swine
- Taurine
(administration & dosage, analogs & derivatives)
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