Preconditioning neuroprotection in global cerebral ischemia involves NMDA receptor-mediated ERK-JNK3 crosstalk.

Previous work has demonstrated that ischemic preconditioning neuroprotection is associated with inhibition of JNK pathway activation. The present study was designed to examine the hypothesis that the suppression of JNK3 activation by preconditioning is mediated by NMDA receptors and crosstalk between ERK1/2 and JNK3. Preconditioning (3 min ischemia) 2 days before global cerebral ischemia (8-min) markedly decreased neuronal degeneration in hippocampus CA1, an effect abolished by pretreatment with the NMDA receptor antagonist, MK-801. Furthermore, preconditioning abolished cerebral ischemia-induced JNK3 activation and enhanced ERK1/2 activation, an effect reversed by MK-801. Due to the inverse relationship between ERK1/2 and JNK3 activation following preconditioning, we hypothesized that ERK1/2 may regulate JNK3 activation following preconditioning. In support of this contention, pretreatment with the MEK inhibitor, PD98059 significantly attenuated preconditioning-induced ERK1/2 phosphorylation, and strongly reversed preconditioning down-regulation of JNK3 phosphorylation. This finding suggests that ERK1/2 signaling is responsible for preconditioning-induced down-regulation of JNK3 activation. Western blot analysis and immunohistochemistry further demonstrated that preconditioning, in an NMDA-dependent manner, enhanced activation of the pro-survival factors, p-CREB and Bcl-2, while attenuating activation of putative pro-death factors, p-c-Jun and Fas-L in the hippocampus CA1. As a whole, the study demonstrates that preconditioning attenuation of pro-death JNK3 in the hippocampus CA1 following global cerebral ischemia is mediated by NMDA receptor-induced crosstalk between ERK1/2 and JNK3. The ERK1/2-mediated reduction of JNK3 activation leads to enhanced pro-survival signaling (P-CREB and Bcl-2 induction) and attenuation of pro-death signaling (p-c-Jun and Fas-L), with subsequent induction of ischemic tolerance.
AuthorsQuan-Guang Zhang, Rui-Min Wang, Dong Han, Li-Cai Yang, Jie Li, Darrell W Brann
JournalNeuroscience research (Neurosci Res) Vol. 63 Issue 3 Pg. 205-12 (Mar 2009) ISSN: 0168-0102 [Print] Ireland
PMID19373993 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
Chemical References
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one
  • Flavonoids
  • Proto-Oncogene Proteins c-bcl-2
  • Receptors, N-Methyl-D-Aspartate
  • Dizocilpine Maleate
  • CREB-Binding Protein
  • Mitogen-Activated Protein Kinase 10
  • Mitogen-Activated Protein Kinase 3
  • Analysis of Variance
  • Animals
  • Brain Ischemia (pathology, prevention & control)
  • CREB-Binding Protein (metabolism)
  • Disease Models, Animal
  • Dizocilpine Maleate (pharmacology)
  • Down-Regulation
  • Enzyme Activation
  • Flavonoids (pharmacology)
  • Gene Expression Regulation, Enzymologic (drug effects)
  • Hippocampus (drug effects, metabolism)
  • In Situ Nick-End Labeling (methods)
  • Ischemic Preconditioning
  • Male
  • Mitogen-Activated Protein Kinase 10 (antagonists & inhibitors, metabolism)
  • Mitogen-Activated Protein Kinase 3 (antagonists & inhibitors, metabolism)
  • Proto-Oncogene Proteins c-bcl-2 (metabolism)
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, N-Methyl-D-Aspartate (antagonists & inhibitors, metabolism)
  • Signal Transduction (drug effects, physiology)
  • Time Factors

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