Potent bactericidal action of a flavonoid fraction isolated from the stem bark of Butea frondosa.

Abstract

The flavonoid fraction isolated from the ethyl acetate fraction (BF-1) of Butea frondosa (L.) stem bark exhibited distinct antimicrobial activity when tested against 129 bacterial strains belonging to 9 different genera of both gram-positive and gram-negative types. Minimum inhibitory concentration (MIC) of the fraction BF-1 was determined following NCCLS guidelines using the agar dilution method. Twenty-four out of 36 strains of Staphylococcus aureus were inhibited by 50-200 mg/l of the fraction. This fraction also inhibited strains of Bacillus spp., Shigella spp., Salmonella spp. and even a few Pseudomonas at concentrations between 50-200 mg/l. Other bacteria including Escherichia coli, Vibrio cholerae and V. parahaemolyticus were moderately sensitive to BF-1. In the in vivo studies, this fraction offered significant protection to Swiss albino mice at a concentration of 80 microg/mouse (p<0.001) when they were challenged with 50 median lethal dose of Salmonella enteritidis NCTC 74. A fraction named BF-1 that was isolated from an ethyl acetate fraction of Butea frondosa provided protection against an infection from a Salmonella enteritidis NCTC strain.

• Authors: U S Mishra, P Chakraborty, A Dasgupta, S G Dastidar, M Martins, L Amaral
• Journal: In vivo (Athens, Greece) (In Vivo) 2009 Jan-Feb Vol. 23 Issue 1 Pg. 29-32 ISSN: 0258-851X [Print] Greece
• PMID: 19368121 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Bacterial Agents
• Flavonoids
• Plant Extracts

Topics

• Animals
• Anti-Bacterial Agents (blood, pharmacology)
• Butea (chemistry)
• Chemical Fractionation
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Flavonoids (blood, pharmacology)
• Gram-Negative Bacteria (drug effects)
• Gram-Positive Bacteria (drug effects)
• Longevity (drug effects)
• Male
• Mice
• Microbial Sensitivity Tests
• Plant Bark (chemistry)
• Plant Extracts (pharmacology)
• Salmonella Infections, Animal (drug therapy, prevention & control)
• Staphylococcus aureus (drug effects)