Mycobacterium tuberculosis PtkA is a novel protein tyrosine kinase whose substrate is PtpA.

Abstract

In Mycobacterium tuberculosis, signal transduction is mediated by 11 serine/threonine kinases, but no tyrosine kinases have been identified thus far. The protein encoded by the ORF (open reading frame) Rv2232 has been annotated as a member of the HAD (haloacid dehydrogenase-like hydrolase) superfamily, which includes phosphatases, phosphomanno- and phosphogluco-mutases, and haloacid dehydrogenases. In the present paper, we report, on the basis of biochemical and mutational analyses, that the Rv2232-encoded protein, named protein tyrosine kinase A (PtkA) is a bona fide protein tyrosine kinase. The cognate substrate of PtkA is the secreted protein tyrosine phosphatase A (PtpA).

• Authors: Horacio Bach, Dennis Wong, Yossef Av-Gay
• Journal: The Biochemical journal (Biochem J) Vol. 420 Issue 2 Pg. 155-60 (May 13 2009) ISSN: 1470-8728 [Electronic] England
• PMID: 19366344 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Proteins
• Tyrosine
• Protein-Tyrosine Kinases
• Receptor-Like Protein Tyrosine Phosphatases, Class 4

Topics

• Amino Acid Substitution
• Bacterial Proteins (genetics, metabolism)
• Binding, Competitive
• Kinetics
• Mutation
• Mycobacterium tuberculosis (enzymology, genetics, metabolism)
• Phosphorylation
• Protein Binding
• Protein-Tyrosine Kinases (genetics, metabolism)
• Receptor-Like Protein Tyrosine Phosphatases, Class 4 (genetics, metabolism)
• Substrate Specificity
• Tyrosine (metabolism)