[Evaluation of serum PIVKA-II by Lumipulse PrestoII assay].
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| Abstract |
Measurements of serum concentrations of Des-gamma-carboxy Prothrombin (PIVKA-II) are widely used for diagnosing hepatocellular carcinoma (HCC). Recently, in Lumipulsef assay, it was reported that antibodies against alkaline phosphatase (ALP) derived from anti bleeding sheets led false high values of PIVKA-II in the patients with HCC resection. To improve the previous issue, newly developed Lumipulse PrestoII assay was examined. (1) The assay was reliable and positively correlated with the previous assays (Lumipulse f and Picolumi, R = 0.997 and 0.994 (n=115), respectively). (2) Eleven cases, which had false high values of PIVKA-II by the Lumipulsef assay, were examined by the PrestoII assay with excess of inactive ALP. The false high values of 10 cases were improved, but only one was still high. False reactivity of this case was stronger than other cases, more effective adsorption was required. (3) Comparing the absorbent activity of inactive ALP among 6 different kinds, we found inactive ALP with much higher adsorbent activity. When this inactive ALP was applied to assay, false high values of PIVKA-II were improved in all 11 cases. In conclusion, the PrestoII assay, which applies the inactive ALP with high activity, is reliable and useful for clinical screening.
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| Authors | Kumiko Hiramatsu, Yasuhito Tanaka, Kazumi Takagi, Satomi Kani, Takaaki Goto, Yoshimitsu Takasaka, Kentaro Matsuura, Fuminaka Sugauchi, Kazushige Moriyama, Hiroshi Murakami, Sachiko Kitajima, Masashi Mizokami |
| Journal | Rinsho byori. The Japanese journal of clinical pathology (Rinsho Byori) Vol. 57 Issue 3 Pg. 200-5 (Mar 2009) ISSN: 0047-1860 [Print] Japan |
| PMID | 19363989 (Publication Type: English Abstract, Journal Article) |
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