Competitive ELISA using a rabies glycoprotein-transformed cell line to semi-quantify rabies neutralizing-related antibodies in dogs.

Abstract

Dogs are the principal vectors for rabies virus transmission to humans in many parts of the world. The current "gold standard" World Health Organization- and Office International des Epizooties-recommended tests for measuring anti-rabies virus antibodies in dogs, such as the fluorescent antibody virus neutralization (FAVN) test, requires high containment facilities and several days for results. Here, we describe a new competitive ELISA (c-ELISA) that utilizes a cell line stably expressing the rabies virus glycoprotein as the coating antigen and two horseradish peroxidase-labeled monoclonal rabies-neutralizing antibodies as indicator, which can be carried out in any basic laboratory. We demonstrate that the c-ELISA has high specificity and sensitivity. Application to 4350 dog serum samples showed a parallel relationship with the FAVN at neutralizing antibody levels up to 8.00 IU/ml. Since it can assay 40 or more samples in 1.5h, the c-ELISA is suitable for the surveillance of mass vaccination in dogs where rabies causes public concern.

• Authors: Shoufeng Zhang, Ye Liu, Fei Zhang, Rongliang Hu
• Journal: Vaccine (Vaccine) Vol. 27 Issue 15 Pg. 2108-13 (Mar 26 2009) ISSN: 0264-410X [Print] Netherlands
• PMID: 19356613 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Antigens, Viral
• Glycoproteins
• Rabies Vaccines
• Viral Envelope Proteins

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antibodies, Viral (blood)
• Antigens, Viral (immunology)
• Cell Line, Transformed
• Dog Diseases (immunology)
• Dogs
• Enzyme-Linked Immunosorbent Assay (methods)
• Glycoproteins (immunology)
• Neutralization Tests
• Rabies (immunology, veterinary)
• Rabies Vaccines (immunology)
• Rabies virus (immunology)
• Sensitivity and Specificity
• Viral Envelope Proteins (immunology)