Normal thiopurine methyltransferase phenotype testing in a Crohn disease patient with azathioprine induced myelosuppression.

Abstract

Severe cytopenias in patients with autoimmune conditions treated with azathioprine are well-recognized. Thiopurine methyltransferase (TPMT) enzymatic activity is subject to individual and ethnic variability. Patients with low TPMT activity (poor metabolizers) are at high risk of developing severe and potentially fatal haematopoietic toxicity. Studies have shown that essentially all TPMT-deficient patients will develop haematopoietic toxicity on administration of conventional thiopurine dosages (6-mercaptopurine, azathioprine). Therefore, screening for TPMT polymorphisms in patients before prescribing thiopurine drugs has been proposed. However, despite normal in vitro enzymatic activity, cytopenia may still occur in vivo. This is the case report of an Asian patient with Crohn disease harbouring a rare TPMT mutation on DNA sequencing, who developed neutropenic sepsis and anaemia after a flare of Crohn disease. The report illustrates the importance of monitoring for cytopenia in the setting of active inflammatory disease despite prior normal phenotyping, the role of predictive pharmacogenetics and the limitations of TPMT phenotype assays that may result in misclassification of at-risk patients.

• Authors: M Leung, I Piatkov, C Rochester, S C Boyages, R W L Leong
• Journal: Internal medicine journal (Intern Med J) Vol. 39 Issue 2 Pg. 121-6 (Feb 2009) ISSN: 1445-5994 [Electronic] Australia
• PMID: 19356188 (Publication Type: Case Reports, Journal Article)

Chemical References

• Methyltransferases
• thiopurine methyltransferase
• Azathioprine

Topics

• Adult
• Azathioprine (adverse effects)
• Bone Marrow Cells (drug effects, enzymology, pathology)
• Crohn Disease (drug therapy, enzymology, genetics)
• Female
• Genetic Testing
• Humans
• Methyltransferases (genetics)
• Phenotype