Characterization of the heat shock protein 90 gene in the plant parasitic nematode Meloidogyne artiellia and its expression as related to different developmental stages and temperature.

Abstract

The full-length cDNA and the corresponding gene of the heat shock protein 90, Mt-Hsp90, were isolated and characterized in the plant parasitic nematode Meloidogyne artiellia. The full-length Mt-Hsp90 cDNA contained a 5' untranslated region (UTR) of 45 bp with the 22 bp trans-spliced leader SL1, an ORF of 2172 bp encoding a polypeptide of 723 amino acids and a 3' UTR of 191 bp. The deduced amino acid sequence of Mt-hsp90 showed high similarity with other known Hsp90s. Five conserved amino acid signatures indicated that Mt-hsp90 is a cytosolic member of the Hsp90 family. The gene consists of 10 exons and 9 introns, a more expanded gene structure compared to the corresponding Caenorhabditis elegans gene, daf-21. Mt-hsp90 gene was constitutively expressed at high levels in all developmental stages of M. artiellia. Egg masses and second stage juveniles (J2s) were exposed at 5 degrees and 30 degrees C for different periods of times in order to explore the impact of adverse temperature on Mt-hsp90 gene expression. Expression levels of Mt-hsp90 were examined by fluorescent real-time PCR. At 30 degrees C a burst of expression for Mt-hsp90 was observed in J2s after 2 h of heat shock treatment, then expression dropped with longer exposing times, although remaining still relatively high after 24 h. This temperature did not affect Mt-hsp90 gene expression in the egg masses. However, egg masses exposed at 5 degrees C showed a little but gradual increase in the mRNA level with time. By contrast, no significant changes in the Mt-hsp90 level were observed in J2s exposed to cold. These data show that egg masses and J2s exposed to cold and heat stresses have different expression profiles suggesting that Mt-Hsp90 may provide a link between environmental conditions and the life cycle of the nematode.

• Authors: Francesca De Luca, Mauro Di Vito, Elena Fanelli, Aurelio Reyes, Nicola Greco, Carla De Giorgi
• Journal: Gene (Gene) Vol. 440 Issue 1-2 Pg. 16-22 (Jul 01 2009) ISSN: 1879-0038 [Electronic] Netherlands
• PMID: 19348876 (Publication Type: Journal Article)

Chemical References

• DNA, Complementary
• HSP90 Heat-Shock Proteins
• Helminth Proteins

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• DNA, Complementary (metabolism)
• Gene Expression Regulation, Developmental
• Genes, Helminth
• HSP90 Heat-Shock Proteins (genetics)
• Helminth Proteins (genetics)
• Introns
• Molecular Sequence Data
• Temperature
• Tylenchoidea (genetics, growth & development, metabolism)