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Cell-cycle regulation and dynamics of cytoplasmic compartments containing the promyelocytic leukemia protein and nucleoporins.

Abstract
Nucleoporins and the promyelocytic leukemia protein (PML) represent structural entities of nuclear pore complexes and PML nuclear bodies, respectively. In addition, these proteins might function in a common biological mechanism, because at least two different nucleoporins, Nup98 and Nup214, as well as PML, can become aberrantly expressed as oncogenic fusion proteins in acute myeloid leukemia (AML) cells. Here we show that PML and nucleoporins become directed to common cytoplasmic compartments during the mitosis-to-G1 transition of the cell cycle. These protein assemblies, which we have termed CyPNs (cytoplasmic assemblies of PML and nucleoporins), move on the microtubular network and become stably connected to the nuclear membrane once contact with the nucleus has been made. The ability of PML to target CyPNs depends on its nuclear localization signal, and loss of PML causes an increase in cytoplasmic-bound versus nuclear-membrane-bound nucleoporins. CyPNs are also targeted by the acute promyelocytic leukemia (APL) fusion protein PML-RARalpha and can be readily detected within the APL cell line NB4. These results provide insight into a dynamic pool of cytoplasmic nucleoporins that form a complex with the tumor suppressor protein PML during the G1 phase of the cell cycle.
AuthorsAsne Jul-Larsen, Amra Grudic, Rolf Bjerkvig, Stig Ove Bøe
JournalJournal of cell science (J Cell Sci) Vol. 122 Issue Pt 8 Pg. 1201-10 (Apr 15 2009) ISSN: 0021-9533 [Print] England
PMID19339552 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Nuclear Localization Signals
  • Nuclear Pore Complex Proteins
  • Nuclear Proteins
  • Oncogene Proteins, Fusion
  • Promyelocytic Leukemia Protein
  • RNA, Small Interfering
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Tubulin Modulators
  • Tumor Suppressor Proteins
  • promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein
  • PML protein, human
  • CREB-Binding Protein
  • CREBBP protein, human
  • Nocodazole
Topics
  • CREB-Binding Protein (metabolism)
  • Cell Cycle (drug effects)
  • Cytoplasm (drug effects, metabolism)
  • G1 Phase
  • HeLa Cells
  • Humans
  • Microtubules (metabolism)
  • Mitosis
  • Nocodazole (pharmacology)
  • Nuclear Envelope (metabolism)
  • Nuclear Localization Signals (metabolism)
  • Nuclear Pore Complex Proteins (metabolism)
  • Nuclear Proteins (genetics, metabolism)
  • Oncogene Proteins, Fusion (metabolism)
  • Promyelocytic Leukemia Protein
  • Protein Transport
  • RNA Interference
  • RNA, Small Interfering (metabolism)
  • Recombinant Fusion Proteins (metabolism)
  • Time Factors
  • Transcription Factors (genetics, metabolism)
  • Transfection
  • Tubulin Modulators (pharmacology)
  • Tumor Suppressor Proteins (genetics, metabolism)

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