Abstract | BACKGROUND: DESIGN AND METHODS: Codanin-1 localization was studied by immunofluorescence and immune electron microscopy. Cell cycle expression of codanin-1 was evaluated using synchronized HeLa cells. E2F proteins are the main regulator of G(1)/S transition. An E2F1-inducible cell line (U20S-ER-E2F1) enabled us to study codanin-1 expression following ectopic E2F1 induction. Direct binding of E2F1 to codanin-1 promoter was assessed by chromatin immunoprecipitation. We used a luciferase-reporter plasmid to study activation of CDAN1 transcription by E2F1. RESULTS: We localized codanin-1 to heterochromatin in interphase cells. During the cell cycle, high levels of codanin-1 were observed in the S phase. At mitosis, codanin-1 underwent phosphorylation, which coincided with its exclusion from condensed chromosomes. The proximal CDAN1 gene promoter region, containing five putative E2F binding sites, was found to be a direct target of E2F1. CONCLUSIONS: Taken together, these data suggest that codanin-1 is a cell cycle-regulated protein active in the S phase. The exact role of codanin-1 during the S phase remains to be determined. Nevertheless this represents the first step towards understanding the function of the proteins involved in congenital dyserythropoietic anemia.
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Authors | Sharon Noy-Lotan, Orly Dgany, Roxane Lahmi, Nathaly Marcoux, Tanya Krasnov, Nissan Yissachar, Doron Ginsberg, Benny Motro, Peretz Resnitzky, Isaac Yaniv, Gary M Kupfer, Hannah Tamary |
Journal | Haematologica
(Haematologica)
Vol. 94
Issue 5
Pg. 629-37
(May 2009)
ISSN: 1592-8721 [Electronic] Italy |
PMID | 19336738
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CDAN1 protein, human
- E2F1 Transcription Factor
- E2F1 protein, human
- Glycoproteins
- Heterochromatin
- Leupeptins
- Nuclear Proteins
- Tamoxifen
- afimoxifene
- Luciferases
- benzyloxycarbonylleucyl-leucyl-leucine aldehyde
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Topics |
- Amino Acid Sequence
- Anemia, Dyserythropoietic, Congenital
(classification, genetics, pathology)
- Base Sequence
- Binding Sites
(genetics)
- Blotting, Western
- Cell Cycle
(physiology)
- Cell Division
(physiology)
- Cell Line, Tumor
- Chromatin Immunoprecipitation
- E2F1 Transcription Factor
(genetics, metabolism)
- G2 Phase
(physiology)
- Gene Expression
(drug effects)
- Glycoproteins
(genetics, metabolism)
- HeLa Cells
- Heterochromatin
(metabolism, ultrastructure)
- Humans
- Leupeptins
(pharmacology)
- Luciferases
(genetics, metabolism)
- Microscopy, Confocal
- Microscopy, Immunoelectron
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- Phosphorylation
- Protein Binding
- Tamoxifen
(analogs & derivatives, pharmacology)
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