Site-related white adipose tissue lipid-handling response to oleoyl-estrone treatment in overweight male rats.

Abstract

BACKGROUND:

Oleoyl-estrone (OE) decreases energy intake while maintaining glucose homeostasis, and energy expenditure at the expense of body fat. White adipose tissue (WAT) depots behave differently under starvation, postprandial state and pharmacologically induced lipolysis.

AIM OF THE STUDY:

To understand the mechanism of massive lipid loss from WAT elicited by OE treatment.

METHODS:

We used overweight male rats. Rats receiving OE (10 nmol/g) gavages were compared with controls and a pair-fed group. Whole fat pads from the mesenteric, retroperitoneal, epididymal and inguinal subcutaneous sites were excised and analyzed for lipid, DNA, mRNA and the expression of lipogenic, fatty acid transporters and lipase genes.

RESULTS:

In OE and pair-fed rats, WAT weights decreased, with the limited loss of cells. Patterns of gene expression in most WAT sites were similar for OE and PF, suggesting a shared mechanism of fat mobilization, but in mesenteric WAT, PF increased lipogenic and fatty acid transporter gene expressions. However, OE inhibited lipogenic expressions more deeply than PF.

CONCLUSIONS:

White adipose tissue sites showed different expression patterns, hinting at relatively specialized functions in fat storage; thus, single site analyses cannot be extrapolated to whole WAT. Differences between mesenteric and the other sites suggest that 'visceral fat' should be reserved for this site only, and not applied to other abdominal fat depots (epididymal, retroperitoneal).

• Authors: María del Mar Romero, José Antonio Fernández-López, Montserrat Esteve, Marià Alemany
• Journal: European journal of nutrition (Eur J Nutr) Vol. 48 Issue 5 Pg. 291-9 (Aug 2009) ISSN: 1436-6215 [Electronic] Germany
• PMID: 19326039 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Obesity Agents
• Oleic Acids
• Estrone
• oleoyl-estrone

Topics

• Adipose Tissue (cytology, drug effects, metabolism)
• Animals
• Anti-Obesity Agents (pharmacology, therapeutic use)
• Body Composition (drug effects)
• Energy Intake (drug effects)
• Estrone (analogs & derivatives, pharmacology, therapeutic use)
• Gene Expression (drug effects)
• Lipid Metabolism (drug effects)
• Lipid Mobilization (drug effects)
• Male
• Oleic Acids (pharmacology, therapeutic use)
• Overweight (drug therapy)
• Random Allocation
• Rats
• Rats, Wistar