In mycobacteria,
F(420), a deazaflavin derivative, acts as a hydride transfer
coenzyme for an F(420)-specific
glucose-6-phosphate dehydrogenase (Fgd). Physiologically relevant reactions in the mycobacteria that use Fgd-generated reduced
F(420) (
F(420)H(2)) are unknown. In this work,
F(420)H(2) was found to be oxidized by NO only in the presence of
oxygen. Further analysis demonstrated that NO(2), produced from NO and O(2), was the
oxidant. UV-visible spectroscopic and NO-sensor-based analyses proved that
F(420)H(2) reduced NO(2) to NO. This reaction could serve as a defense system for Mycobacterium tuberculosis, which is more sensitive to NO(2) than NO under aerobic conditions. Activated macrophages produce NO, which in acidified phagosomes is converted to NO(2). Hence, by converting NO(2) back to NO with
F(420)H(2), M.
tuberculosis could decrease the effectiveness of antibacterial action of macrophages; such defense would correspond to active
tuberculosis conditions where the bacterium grows aerobically. This hypothesis was consistent with the observation that a mutant strain of Mycobacterium smegmatis, a nonpathogenic relative of M.
tuberculosis, which either did not produce or could not reduce
F(420), was approximately 4-fold more sensitive to NO(2) than the wild-type strain. The phenomenon is reminiscent of the anticancer activity of
gamma-tocopherol, which reduces NO(2) to NO and protects human cells from NO(2)-induced
carcinogenesis.