In vitro dopaminergic neuroprotective and in vivo antiparkinsonian-like effects of Delta 3,2-hydroxybakuchiol isolated from Psoralea corylifolia (L.).

Abstract

Cocktail recipes containing Psoralea corylifolia seeds (PCS) are used to empirically treat Parkinson disease. A PCS isolate Delta(3),2-hydroxybakuchiol (BU) can inhibit dopamine uptake in dopamine transporter (DAT) transfected Chinese hamster ovary (CHO) cells, and dopamine reuptake blockade may provide an alternative approach for ameliorating parkinsonism. Here, we assessed the potential dopaminergic neuroprotective, and antiparkinsonian-like activity of BU. BU sample size was increased by using a scale-up extraction paradigm. Pharmacologically, BU significantly protected SK-N-SH cells from 1-methyl-4-phenylpyridinium (MPP(+)) insult, produced striking inhibitory actions on dopamine/norepinephrine uptake and WIN35,428 binding in synaptosomes on in vivo administration, and significantly preventing poor performance on rotarod and dopaminergic loss in substantia nigra in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mice. BU acts by protecting dopaminergic neurons from MPP(+) injury and preventing against MPTP-induced behavioral and histological lesions in the Parkinson's disease (PD) model, possibly by inhibiting monoamine transporters. These findings suggest that BU could be meaningful in PD treatment.

• Authors: G Zhao, X-W Zheng, G-W Qin, Y Gai, Z-H Jiang, L-H Guo
• Journal: Cellular and molecular life sciences : CMLS (Cell Mol Life Sci) Vol. 66 Issue 9 Pg. 1617-29 (May 2009) ISSN: 1420-9071 [Electronic] Switzerland
• PMID: 19322517 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiparkinson Agents
• Dopamine Agents
• Neuroprotective Agents
• Phenols
• delta(3),2-hydroxybakuchiol
• (1R-(exo,exo))-3-(4-fluorophenyl)-8-methyl-8- azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester
• 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
• Tyrosine 3-Monooxygenase
• Cocaine
• 1-Methyl-4-phenylpyridinium
• Dopamine
• Norepinephrine

Topics

• 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (pharmacology)
• 1-Methyl-4-phenylpyridinium (pharmacology)
• Animals
• Antiparkinson Agents (chemistry, isolation & purification, pharmacology)
• CHO Cells
• Cell Line
• Cocaine (analogs & derivatives, metabolism)
• Cricetinae
• Cricetulus
• Dopamine (metabolism)
• Dopamine Agents (chemistry, isolation & purification, pharmacology)
• Humans
• Male
• Mice
• Mice, Inbred C57BL
• Neurons (drug effects, metabolism)
• Neuroprotective Agents (chemistry, isolation & purification, pharmacology)
• Norepinephrine (metabolism)
• Parkinson Disease (drug therapy)
• Phenols (chemistry, isolation & purification, pharmacology)
• Psoralea (chemistry)
• Rats
• Rats, Sprague-Dawley
• Synaptosomes (drug effects, metabolism)
• Tyrosine 3-Monooxygenase (metabolism)