Histatins, a family of
salivary proteins, have antimicrobial activity. Candida albicans, which is killed by
histatins, induces
oral candidiasis in individuals with compromised immune systems. Although the functional significance of
histatins has been documented, their
biological and physiological functions against host cells have not been clarified. In this study, we found that
histatin 3, a member of the
histatin family, binds to heat shock cognate
protein 70 (HSC70). These
proteins were co-localized in the cytoplasm and nucleus in human gingival fibroblasts following non-heat and heat shock.
Histatin 3 induced stimulation of
DNA synthesis and cell survival in human gingival fibroblasts in a dose-dependent manner. This
DNA synthesis was found to be dependent on HSC70 by knockdown experiments. The effect of heat shock on
DNA synthesis induced by
histatin 3 was approximately 2-fold higher than that of non-heat shock. When the
histatin 3 uptake into cells was inhibited by
monodansylcadaverine or when
histatin 3 binding to HSC70 was precluded by
15-deoxyspergualin,
DNA synthesis by
histatin 3 was approximately 2-fold less than that without
monodansylcadaverine or
15-deoxyspergualin. Although HSC70 directly bound to p27(Kip1) (a
cyclin-dependent kinase inhibitor),
histatin 3 increased the binding between those
proteins but not with a
peptide capable of binding to HSC70. Moreover
histatin 3 prevented
ATP-dependent dissociation of HSC70-p27(Kip1).
ATP was unable to form a
histatin 3-HSC70(D10N)-p27(Kip1) complex (HSC70(D10N) is a mutant attenuating
ATPase activity). These findings suggest that
histatin 3 may be involved in cell proliferation through the regulation of HSC70 and p27(Kip1) in oral cells.