Transforming growth factor-B1 and matrix metalloproteinase-7 promoter variants induce risk for Helicobacter pylori-associated gastric precancerous lesions.

Abstract

The expression of growth factors, proteolytic enzymes, fibrogenic factors, and cytokines is altered in the Helicobacter pylori-infected gastric mucosa. Therefore, we aimed to evaluate the association of functional promoter variants of transforming growth factor (TGF)-B1 and matrix metalloproteinase (MMP)-7 genes with gastritis and gastric precancerous lesions. After upper gastrointestinal endoscopy, a total of 130 rapid urease test-positive patients with nonulcer dyspepsia were examined for H. pylori infection using modified Giemsa stain and IgG anti-CagA ELISA. All patients and 200 asymptomatic controls were genotyped for TGF-B1 (-509 C>T) and MMP-7 (-181 A>G) substitutions using PCR-RFLP. The genotype and allele frequencies of TGF-B1 and MMP-7 polymorphisms did not differ between patients and controls (p > 0.05). However, the CagA-positive patients with TGF-B1 -509 T allele had higher risk for gastric atrophy (p = 0.026, odds ratio [OR] = 2.38) and lymphoid follicle development (p = 0.028, OR = 2.29). In addition, CagA-positive patients carrying MMP-7 -181 G allele had risk for lymphoid follicle formation (p = 0.027, OR = 2.30). Thus, the present study revealed significant association of functional MMP-7 and TGF-B1 gene variants toward susceptibility to H. pylori-induced precancerous gastric lesions.

• Authors: B R Achyut, Uday C Ghoshal, Nikhil Moorchung, Balraj Mittal
• Journal: DNA and cell biology (DNA Cell Biol) Vol. 28 Issue 6 Pg. 295-301 (Jun 2009) ISSN: 1557-7430 [Electronic] United States
• PMID: 19317620 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial
• Bacterial Proteins
• Transforming Growth Factor beta1
• cagA protein, Helicobacter pylori
• MMP7 protein, human
• Matrix Metalloproteinase 7

Topics

• Adult
• Alleles
• Antigens, Bacterial (analysis)
• Atrophy
• Bacterial Proteins (analysis)
• Female
• Gastritis (genetics, pathology)
• Genotype
• Helicobacter Infections (genetics, pathology)
• Helicobacter pylori
• Humans
• Lymphoid Tissue (pathology)
• Male
• Matrix Metalloproteinase 7 (genetics)
• Middle Aged
• Polymorphism, Single Nucleotide
• Precancerous Conditions (genetics, microbiology, pathology)
• Promoter Regions, Genetic (genetics)
• Risk
• Sequence Deletion
• Stomach (pathology)
• Stomach Neoplasms (epidemiology, microbiology)
• Transforming Growth Factor beta1 (genetics)