Imaging neural stem cell fate in mouse model of glioma.

Abstract

This unit describes a protocol for following the fate of stem cells in real time in a mouse model of glioma. Stem cells and tumor cells can be transduced with lentiviral vectors bearing two different luciferases, firefly luciferase (Fluc) and Renilla (Rluc) luciferase, respectively. With the cells labeled in this manner, bioluminescence imaging can be used to study the fate of stem cells in glioma-bearing brains in vivo.

• Authors: Khalid Shah
• Journal: Current protocols in stem cell biology (Curr Protoc Stem Cell Biol) Vol. Chapter 5 Pg. Unit 5A.1 (Mar 2009) ISSN: 1938-8969 [Electronic] United States
• PMID: 19306259 (Publication Type: Journal Article)
• Copyright: Copyright 2009 by John Wiley & Sons, Inc.

Chemical References

• Luciferases, Renilla
• Luciferases, Firefly

Topics

• Animals
• Diagnostic Imaging (methods)
• Disease Models, Animal
• Genetic Vectors
• Glioma (pathology)
• Lentivirus (genetics)
• Luciferases, Firefly
• Luciferases, Renilla
• Luminescent Measurements
• Methods
• Mice
• Neurons (pathology)
• Stem Cells (pathology)
• Transduction, Genetic