Identification of an invasive infection of R. oryzae in a haematological patient using a molecular technique.

We report a case of cerebral mucormycosis in a 28-year-old male who was affected by chronic myeloid leukaemia and underwent allogeneic bone marrow transplantation. Nine months post-transplantation, he was admitted to the hospital with fever, bilateral eyelid oedema and neutropenia. X-ray analysis showed numerous areas of pulmonary parenchymal thickening, and a computed tomography scan of the brain showed inflammation of the frontal, maxillary, ethmoidal and sphenoidal sinuses and diffuse swelling of the periorbital tissues. Sinus cultures were taken, and based on its characteristic rhizoid structure, we classified the isolated fungus as a member of the genus Rhizopus. The fungus was identified as an Rhizopus oryzae species, as assessed by sequencing of the internal transcribed spacer of the rRNA gene. Treatment with amphotericin B was ineffective, however, and the patient died 2 weeks after admission. This case highlights the potential severity of an invasive infection of R. oryzae, identified by molecular biology techniques.
AuthorsFrancesca Cavrini, Marta Stanzani, Giovanna Liguori, Vittorio Sambri
JournalMycoses (Mycoses) Vol. 53 Issue 3 Pg. 269-71 (May 2010) ISSN: 1439-0507 [Electronic] Germany
PMID19302460 (Publication Type: Case Reports, Letter)
Chemical References
  • DNA, Fungal
  • DNA, Ribosomal
  • DNA, Ribosomal Spacer
  • Adult
  • Bone Marrow Transplantation
  • Brain (microbiology, radiography)
  • Central Nervous System Fungal Infections (diagnosis, microbiology)
  • DNA, Fungal (chemistry, genetics)
  • DNA, Ribosomal (chemistry, genetics)
  • DNA, Ribosomal Spacer (chemistry, genetics)
  • Humans
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive (complications)
  • Lung (pathology)
  • Male
  • Mucormycosis (diagnosis, microbiology)
  • Radiography, Thoracic
  • Rhizopus (isolation & purification)
  • Sequence Analysis, DNA
  • Tomography

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