Cytogenetic investigation of chromosomal aberrations in cells treated with plantamajoside from Plantago asiatica.

Abstract

Plantago asiatica is a member of the Plantaginaceae family, and is widely distributed in East Asia. In our previous work, a single active compound, plantamajoside was isolated and confirmed to have glycation inhibitory activity, and did not possess toxicity during a 90 day repeated oral toxicity test in rats. In the present study, a chromosomal aberration test was performed to investigate the genotoxicity of plantamajoside. From the results of the cytotoxicity test, plantamajoside proved to be less toxic when it was treated combined with S9 cell fractions. However, there was a significant increase in structural aberrations during the short-term treatment of plantamajoside at its highest dose (5000 microg/mL) even when combined with S9. This seems to have been a natural phenomenon due to the very high dose of plantamajoside that was used. However, to confirm the safety of plantamajoside for its potential use as a phytochemical agent in health products, additional mutagenicity tests are necessary.

• Authors: Yun-Chang Koo, Sung-Hoon Jung, Ji-Hee Yang, Yung-Sun Ryu, Eun-Jin Kim, Kwang-Won Lee
• Journal: Phytotherapy research : PTR (Phytother Res) Vol. 23 Issue 10 Pg. 1479-81 (Oct 2009) ISSN: 1099-1573 [Electronic] England
• PMID: 19288521 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: (c) 2009 John Wiley & Sons, Ltd.

Chemical References

• Catechols
• Glucosides
• Plant Extracts
• plantamajoside

Topics

• Animals
• Biotransformation (genetics)
• Catechols (isolation & purification, toxicity)
• Cell Line
• Chromosome Aberrations
• Chromosomes (drug effects)
• Cricetinae
• Cricetulus
• Dose-Response Relationship, Drug
• Fibroblasts (drug effects)
• Glucosides (isolation & purification, toxicity)
• Plant Extracts (chemistry, toxicity)
• Plantago (chemistry)