The protein expression of TRP-1 and galectin-1 in cutaneous malignant melanomas.

Abstract

BACKGROUND:

Patients with metastazing malignant melanoma have a poor outcome and determination of thickness of the primary tumor remains as the most important prognostic predictor. The aim of this study was to use an antibody-based proteomics strategy to search for new molecular markers associated with melanoma progression. Two proteins, TRP-1 and galectin-1, were identified as proteins with enhanced expression in cells from the melanocytic lineage.

PATIENTS AND METHODS:

Protein profiling of TRP-1 and galectin-1 together with proliferation marker Ki-67 and melanocyte marker Melan-A was performed in normal tissues from 144 individuals and in 216 different tumors using tissue microarrays and immunohistochemistry. The protein expression pattern was further analyzed in a defined cohort of 157 patients diagnosed with invasive cutaneous malignant melanoma.

RESULTS:

Both TRP-1 and galectin-1 were highly expressed in normal melanocytes and melanoma. The expression of TRP-1 was inversely correlated with tumor stage (p=0.002, (R=-0.28)). Neither TRP-1 or galectin-1 was associated with overall or disease free survival (p>0.14, p>0.46 respectively). Ki-67 was associated with tumor stage and survival (p<0.001).

CONCLUSION:

TRP-1 and galectin-1 protein expression patterns were determined in normal and cancer tissues and both proteins were expressed in the majority of the malignant melanomas. There was no correlation between TRP-1 or galectin-1 expression and survival.

• Authors: Asa Bolander, Margrét Agnarsdóttir, Sara Strömberg, Fredrik Ponten, Patrik Hesselius, Mathias Uhlen, Michael Bergqvist
• Journal: Cancer genomics & proteomics (Cancer Genomics Proteomics) 2008 Nov-Dec Vol. 5 Issue 6 Pg. 293-300 ISSN: 1109-6535 [Print] Greece
• PMID: 19287070 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Galectin 1
• Ki-67 Antigen
• LGALS1 protein, human
• Membrane Glycoproteins
• Neoplasm Proteins
• Oxidoreductases
• TYRP1 protein, human

Topics

• Disease-Free Survival
• Female
• Follow-Up Studies
• Galectin 1 (biosynthesis)
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic
• Humans
• Ki-67 Antigen (biosynthesis)
• Male
• Melanocytes
• Melanoma (metabolism, mortality)
• Membrane Glycoproteins (biosynthesis, metabolism)
• Neoplasm Invasiveness
• Neoplasm Metastasis
• Neoplasm Proteins (biosynthesis)
• Oxidoreductases (biosynthesis, metabolism)
• Retrospective Studies
• Skin Neoplasms (metabolism, mortality)
• Survival Rate