Globins synthesize the second messenger bis-(3'-5')-cyclic diguanosine monophosphate in bacteria.

Abstract

Globin-coupled sensors are heme-binding signal transducers in Bacteria and Archaea in which an N-terminal globin controls the activity of a variable C-terminal domain. Here, we report that BpeGReg, a globin-coupled diguanylate cyclase from the whooping cough pathogen Bordetella pertussis, synthesizes the second messenger bis-(3'-5')-cyclic diguanosine monophosphate (c-di-GMP) upon oxygen binding. Expression of BpeGReg in Salmonella typhimurium enhances biofilm formation, while knockout of the BpeGReg gene of B. pertussis results in decreased biofilm formation. These results represent the first identification a signal ligand for any diguanylate cyclase and provide definitive experimental evidence that a globin-coupled sensor regulates c-di-GMP synthesis and biofilm formation. We propose that the synthesis of c-di-GMP by globin sensors is a widespread phenomenon in bacteria.

• Authors: Xuehua Wan, Jason R Tuckerman, Jennifer A Saito, Tracey Allen K Freitas, James S Newhouse, Judith R Denery, Michael Y Galperin, Gonzalo Gonzalez, Marie-Alda Gilles-Gonzalez, Maqsudul Alam
• Journal: Journal of molecular biology (J Mol Biol) Vol. 388 Issue 2 Pg. 262-70 (May 01 2009) ISSN: 1089-8638 [Electronic] Netherlands
• PMID: 19285985 (Publication Type: Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Escherichia coli Proteins
• bis(3',5')-cyclic diguanylic acid
• Globins
• Phosphorus-Oxygen Lyases
• diguanylate cyclase
• Cyclic GMP
• Oxygen

Topics

• Bacteria (metabolism)
• Biofilms (growth & development)
• Bordetella pertussis (enzymology)
• Cyclic GMP (analogs & derivatives, biosynthesis)
• Escherichia coli Proteins
• Globins (physiology)
• Oxygen (metabolism)
• Phosphorus-Oxygen Lyases (metabolism)
• Salmonella typhimurium (enzymology)
• Second Messenger Systems
• Signal Transduction