Evaluation of (64)Cu(DO3A-xy-TPEP) as a potential PET radiotracer for monitoring tumor multidrug resistance.

Abstract

In this study, we evaluated the potential of (64)Cu(DO3A-xy-TPEP) (DO3A-xy-TPEP = (2-(diphenylphosphoryl)ethyl)diphenyl(4-((4,7,10-tris(carboxymethyl)-1,4,7,10-tetraazacyclododecan-1-yl)methyl)benzyl)phosphonium) as a PET (positron emission tomography) radiotracer for noninvasive monitoring of multidrug resistance (MDR) transport function in several xenografted tumor models (MDR-negative: U87MG; MDR-positive: MDA-MB-435, MDA-MB-231, KB-3-1, and KB-v-1). It was found that (64)Cu(DO3A-xy-TPEP) has a high initial tumor uptake (5.27 +/- 1.2%ID/g at 5 min p.i.) and shows a steady uptake increase between 30 and 120 min p.i. (2.09 +/- 0.53 and 3.35 +/- 1.27%ID/g at 30 and 120 min p.i., respectively) in the MDR-negative U87MG glioma tumors. (64)Cu(DO3A-xy-TPEP) has a greater uptake difference between U87MG glioma and MDR-positive tumors (MDA-MB-231: 1.57 +/- 0.04, 1.00 +/- 0.17, and 0.93 +/- 0.15; MDA-MB-435: 1.15 +/- 0.19, 1.12 +/- 0.20, and 0.81 +/- 0.11; KB-3-1: 1.45 +/- 0.31, 1.43 +/- 0.16, and 1.08 +/- 0.19; and KB-v-1: 1.63 +/- 0.47, 1.81 +/- 0.31, and 1.14 +/- 0.22%ID/g at 30, 60, and 120 min p.i., respectively) than (99m)Tc-Sestamibi. Regardless of the source of MDR, the overall net effect is the rapid efflux of (64)Cu(DO3A-xy-TPEP) from tumor cells, which leads to a significant reduction of its tumor uptake. It was concluded that (64)Cu(DO3A-xy-TPEP) is more efficient than (99m)Tc-Sestamibi as the substrate for MDR P-glycoproteins (MDR Pgps) and multidrug resistance-associated proteins (MRPs), and might be a more efficient radiotracer for noninvasive monitoring of the tumor MDR transport function. (64)Cu(DO3A-xy-TPEP) and (99m)Tc-Sestamibi share almost identical subcellular distribution patterns in U87MG glioma tumors. Thus, it is reasonable to believe that (64)Cu(DO3A-xy-TPEP), like (99m)Tc-Sestamibi, is able to localize in mitochondria due to the increased plasma and mitochondrial transmembrane potentials in tumor cells.

• Authors: Shuang Liu, Young-Seung Kim, Shizhen Zhai, Jiyun Shi, Guihua Hou
• Journal: Bioconjugate chemistry (Bioconjug Chem) Vol. 20 Issue 4 Pg. 790-8 (Apr 2009) ISSN: 1520-4812 [Electronic] United States
• PMID: 19284752 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B
• Multidrug Resistance-Associated Proteins
• Organometallic Compounds
• Organophosphorus Compounds
• Radioactive Tracers
• Copper
• Cyclosporine

Topics

• ATP Binding Cassette Transporter, Subfamily B (metabolism)
• Animals
• Blotting, Western
• Cell Line, Tumor
• Copper (chemistry)
• Cyclosporine (pharmacology)
• Drug Resistance, Multiple
• Drug Resistance, Neoplasm
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Intracellular Space (metabolism)
• Liver (drug effects, metabolism)
• Metabolic Clearance Rate
• Mice
• Multidrug Resistance-Associated Proteins (metabolism)
• Neoplasms (diagnostic imaging, genetics, metabolism, pathology)
• Organometallic Compounds (chemistry, metabolism, pharmacokinetics)
• Organophosphorus Compounds (metabolism, pharmacokinetics)
• Positron-Emission Tomography
• Radioactive Tracers
• Tissue Distribution