Abstract | PURPOSE: METHODS: RESULTS: A murine interleukin-10 producing S. typhimurium model was constructed. Enzyme linked immunosorbent assay and mast cell bioassay revealed interleukin-10 production. After single oral gavage feeding of 10 bacteria, persistence was noted within mesenteric lymph nodes at 6 weeks. Inoculation with/without the interleukin-10 plasmid (n = 7 per group) was performed before and after dextran sodium sulfate exposure. Postdextran sodium sulfate treatment revealed enhanced weight recovery in the S. typhimurium/ interleukin-10 group compared to S. typhimurium/plasmid and phosphate buffered saline controls (P < 0.0001). The mean histology score for S. typhimurium/ interleukin-10 was 0.86 compared to 3.14 and 3.17 for the S. typhimurium/plasmid and phosphate buffered saline controls respectively (P = 0.028). CONCLUSIONS: Attenuated S. typhimurium producing interleukin-10 can be successfully delivered to the murine gastrointestinal tract by single oral dosing. This novel delivery method improved recovery of chemically-induced murine colitis.
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Authors | Jinggang Lan, Thomas F Warner, Charles P Heise |
Journal | Diseases of the colon and rectum
(Dis Colon Rectum)
Vol. 52
Issue 2
Pg. 230-8
(Feb 2009)
ISSN: 1530-0358 [Electronic] United States |
PMID | 19279417
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Immunologic Factors
- Interleukin-10
- Dextran Sulfate
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Topics |
- Administration, Oral
- Animals
- Colitis
(chemically induced, pathology, therapy)
- Dextran Sulfate
- Gastrointestinal Tract
(microbiology)
- Genetic Vectors
- Immunologic Factors
(administration & dosage, biosynthesis)
- Interleukin-10
(administration & dosage, biosynthesis, genetics)
- Male
- Mice
- Mice, Inbred C57BL
- Microbial Sensitivity Tests
- Salmonella typhimurium
(drug effects, growth & development, metabolism)
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