Abstract | BACKGROUND: Clinical observations are suggesting accelerated granulation tissue formation in traumatic wounds treated with vacuum-assisted closure (VAC). Aim of this study was to determine the impact of VAC therapy versus alternative Epigard application on local inflammation and neovascularization in traumatic soft tissue wounds. METHODS: RESULTS: All cytokines were found to be elevated in wound fluids during both VAC and Epigard treatment, whereas serum concentrations were negligible or not detectable. In wound fluids, significantly higher IL-8 (p < 0.001) and VEGF (p < 0.05) levels were detected during VAC therapy. Furthermore, histologic examination revealed increased neovascularization (p < 0.05) illustrated by CD31 and von Willebrand factor immunohistochemistry in wound biopsies of VAC treatment. In addition, there was an accumulation of neutrophils as well as an augmented expression of VEGF (p < 0.005) in VAC wound biopsies. CONCLUSION: This study suggests that VAC therapy of traumatic wounds leads to increased local IL-8 and VEGF concentrations, which may trigger accumulation of neutrophils and angiogenesis and thus, accelerate neovascularization.
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Authors | Ludwig Labler, Mario Rancan, Ladislav Mica, Luc Härter, Daniela Mihic-Probst, Marius Keel |
Journal | The Journal of trauma
(J Trauma)
Vol. 66
Issue 3
Pg. 749-57
(Mar 2009)
ISSN: 1529-8809 [Electronic] United States |
PMID | 19276749
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fluorocarbon Polymers
- Interleukin-6
- Interleukin-8
- Platelet Endothelial Cell Adhesion Molecule-1
- Vascular Endothelial Growth Factor A
- von Willebrand Factor
- Fibroblast Growth Factor 2
- polytetrafluoroethane
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Topics |
- Adult
- Amputation, Traumatic
(immunology, pathology, therapy)
- Arm Injuries
(immunology, pathology, therapy)
- Biopsy
- Female
- Fibroblast Growth Factor 2
(blood)
- Fluorocarbon Polymers
- Fractures, Open
(immunology, pathology, therapy)
- Humans
- Injury Severity Score
- Interleukin-6
(blood)
- Interleukin-8
(blood)
- Leg Injuries
(immunology, pathology, therapy)
- Leukocyte Count
- Male
- Middle Aged
- Negative-Pressure Wound Therapy
- Neovascularization, Pathologic
(immunology, pathology)
- Neutrophils
(immunology)
- Platelet Endothelial Cell Adhesion Molecule-1
(blood)
- Skin Transplantation
- Soft Tissue Injuries
(immunology, pathology, therapy)
- Surgical Flaps
- Vascular Endothelial Growth Factor A
(blood)
- Wound Healing
(immunology)
- Wounds and Injuries
(immunology, pathology, therapy)
- von Willebrand Factor
(metabolism)
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