Muscovite reverses gastric gland atrophy and intestinal metaplasia by promoting cell proliferation in rats with atrophic gastritis.

Abstract

OBJECTIVE:

To detect whether muscovite exerts its protective role in the progression of atrophic gastritis (AG) and evaluate the possible mechanism.

METHODS:

AG rats were established and then randomly divided into groups administrated with different doses of muscovite for 8 weeks. Histological changes in gastric antrum and the number of parietal cells, chief cells, and G/D cells were observed. Serum gastrin and prostaglandin E2 (PGE2) were evaluated by enzyme-linked immunosorbent assay (ELISA). The expression of proliferating cell nuclear antigen (PCNA), epidermal growth factor receptor (EGFR), C-erbB-2, p21, p53, p16 and bcl-2 in gastric tissue were detected by immunohistochemistry. The concentrations of TNF-alpha and IL-1beta secreted by THP-1 cells were detected when incubated with different doses of muscovite.

RESULTS:

The grade of inflammation in muscovite groups was lower (p < 0.05), while the thickness and number of gastric glands were significantly elevated in muscovite groups (p < 0.01). The expression height of PCNA in the muscovite group was higher than in the AG group (p < 0.01). Immunohistochemistry results showed a positive expression rate of EGFR; p16 in muscovite-treated AG rats was increased (p < 0.05), while C-erbB-2 and p21 were decreased (p < 0.05). Serum gastrin and PGE2 were significantly elevated in the high-dose muscovite-treated rats (p < 0.05). In vitro studies showed that muscovite had a dose-dependent adsorption of TNF-alpha and IL-1beta.

CONCLUSION:

Muscovite could reverse gastric gland atrophy and intestinal metaplasia by promoting cell proliferation and revitalization in gastric mucosa in AG rats.

• Authors: Liang-jing Wang, Qun-yan Zhou, Yan Chen, Shu-jie Chen, Miao Xu, Qin Du, Fang-shi Zhu, Jian-min Si
• Journal: Digestion (Digestion) Vol. 79 Issue 2 Pg. 79-91 ( 2009) ISSN: 1421-9867 [Electronic] Switzerland
• PMID: 19276636 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 S. Karger AG, Basel.

Chemical References

• Aluminum Silicates
• Gastrins
• Interleukin-1beta
• Proliferating Cell Nuclear Antigen
• Tumor Necrosis Factor-alpha
• muscovite
• Egfr protein, rat
• ErbB Receptors
• Receptor, ErbB-2
• Dinoprostone

Topics

• Adsorption
• Aluminum Silicates (chemistry, pharmacology, therapeutic use)
• Animals
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Dinoprostone (blood)
• ErbB Receptors (metabolism)
• Gastric Mucosa (drug effects, metabolism, pathology, ultrastructure)
• Gastrins (blood)
• Gastritis, Atrophic (blood, drug therapy, pathology)
• Humans
• Interleukin-1beta (analysis)
• Male
• Metaplasia (drug therapy, pathology)
• Proliferating Cell Nuclear Antigen (metabolism)
• Rats
• Rats, Sprague-Dawley
• Receptor, ErbB-2 (metabolism)
• Regeneration (drug effects)
• Tumor Necrosis Factor-alpha (analysis)