Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways.

Abstract

BACKGROUND:

Synthetic peptides containing the RGD sequence inhibit integrin-related functions in different cell systems. Here, we investigated the effects of synthetic Arg-Gly-Asp-Ser (RGDS) peptide on key inflammatory responses to intratracheal (i.t.) lipopolysaccharide (LPS) treatment and on the integrin signaled mitogen-activated protein (MAP) kinase pathway during the development of acute lung injury.

METHODS:

Saline or LPS (1.5 mg/kg) was administered i.t. with or without a single dose of RGDS (1, 2.5, or 5 mg/kg, i.p.), anti-alphav or anti-beta3 mAb (5 mg/kg, i.p.). Mice were sacrificed 4 or 24 h post-LPS.

RESULTS:

A pretreatment with RGDS inhibited LPS-induced increases in neutrophil and macrophage numbers, total protein levels and TNF-alpha and MIP-2 levels, and matrix metalloproteinase-9 activity in bronchoalveolar lavage (BAL) fluid at 4 or 24 h post-LPS treatment. RGDS inhibited LPS-induced phosphorylation of focal adhesion kinase and MAP kinases, including ERK, JNK, and p38 MAP kinase, in lung tissue. Importantly, the inhibition of the inflammatory responses and the kinase pathways were still evident when this peptide was administered 2 h after LPS treatment. Similarly, a blocking antibody against integrin alphav significantly inhibited LPS-induced inflammatory cell migration into the lung, protein accumulation and proinflammatory mediator production in BAL fluid, at 4 or 24 h post-LPS. Anti-beta3 also inhibited all LPS-induced inflammatory responses, except the accumulation of BAL protein at 24 h post-LPS.

CONCLUSION:

These results suggest that RGDS with high specificity for alphavintegrins attenuates inflammatory cascade during LPS-induced development of acute lung injury.

• Authors: Changsuk Moon, Jeong Ran Han, Hyun-Jung Park, Jong Sik Hah, Jihee Lee Kang
• Journal: Respiratory research (Respir Res) Vol. 10 Pg. 18 (Mar 09 2009) ISSN: 1465-993X [Electronic] England
• PMID: 19272161 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chemokine CXCL2
• Cxcl2 protein, mouse
• Inflammation Mediators
• Integrin alphaVbeta3
• Lipopolysaccharides
• Oligopeptides
• Tumor Necrosis Factor-alpha
• lipopolysaccharide, E coli O55-B5
• RGES peptide
• arginyl-glycyl-aspartyl-serine
• Focal Adhesion Kinase 1
• Ptk2 protein, mouse
• Extracellular Signal-Regulated MAP Kinases
• JNK Mitogen-Activated Protein Kinases
• p38 Mitogen-Activated Protein Kinases
• Matrix Metalloproteinase 9
• Mmp9 protein, mouse

Topics

• Acute Lung Injury (chemically induced, enzymology, immunology, prevention & control)
• Animals
• Bronchoalveolar Lavage Fluid (immunology)
• Chemokine CXCL2 (metabolism)
• Chemotaxis (drug effects)
• Disease Models, Animal
• Extracellular Signal-Regulated MAP Kinases (metabolism)
• Focal Adhesion Kinase 1 (metabolism)
• Inflammation Mediators (metabolism)
• Integrin alphaVbeta3 (metabolism)
• JNK Mitogen-Activated Protein Kinases (metabolism)
• Lipopolysaccharides
• Lung (drug effects, enzymology, immunology)
• MAP Kinase Signaling System (drug effects)
• Macrophages (drug effects, immunology)
• Male
• Matrix Metalloproteinase 9 (metabolism)
• Mice
• Mice, Inbred BALB C
• Neutrophil Infiltration (drug effects)
• Oligopeptides (pharmacology)
• Phosphorylation
• Pneumonia (chemically induced, enzymology, immunology, prevention & control)
• Time Factors
• Tumor Necrosis Factor-alpha (metabolism)
• p38 Mitogen-Activated Protein Kinases (metabolism)