Structural identification and cytotoxic activity of a polysaccharide from the fruits of Lagenaria siceraria (Lau).

Abstract

A water-soluble polysaccharide, isolated from fruiting bodies of Lagenaria siceraria, is composed of methyl-alpha-d-galacturonate, 3-O-acetyl methyl-alpha-d-galacturonate, and beta-d-galactose in a ratio of nearly 1:1:1. Compositional analysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, 2D-COSY, TOCSY, NOESY, HMQC, and HMBC) revealed the presence of the following repeating unit in the polysaccharide: [carbohydrate structure: see text] This polysaccharide showed cytotoxic activity in vitro against human breast adenocarcinoma cell line (MCF-7).

• Authors: Kaushik Ghosh, Krishnendu Chandra, Arnab K Ojha, Siddik Sarkar, Syed S Islam
• Journal: Carbohydrate research (Carbohydr Res) Vol. 344 Issue 5 Pg. 693-8 (Mar 31 2009) ISSN: 1873-426X [Electronic] Netherlands
• PMID: 19264294 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Polysaccharides

Topics

• Carbohydrate Sequence
• Cell Line, Tumor
• Cell Survival (drug effects)
• Cucurbitaceae (chemistry)
• Fruit (chemistry)
• Humans
• Magnetic Resonance Spectroscopy
• Molecular Sequence Data
• Polysaccharides (chemistry, isolation & purification, pharmacology)