Tumor-associated carbohydrate antigens: Sialyl Lea and T/Tn antigens in canine mammary tumors.

Abstract

Twenty-eight canine mammary tubulopapillary carcinomas and 14 simple adenomas were studied by immunohistochemistry for the expressions of the tumor-associated carbohydrate antigens. Sialyl Le(a) was detected in 71.42% of the malignant and 92.84% of the benign tumors. Staining with anti-T and anti-Tn monoclonal antibodies revealed that 85.70% of the tubulopapillary carcinomas expressed T and Tn antigens. In contrast, 50% of the adenomas did not express T antigen, and 42.85% of them were only weakly stained for this carbohydrate structure. In the case of Tn antigen, the majority (57.14%) of samples was weakly stained, and no binding was observed in 35.71% of the analyzed specimens. Comparison of average values of reaction intensity (IRS) scale for malignant versus benign tumors by the Mann-Whitney U-test revealed a significant relationship between T and Tn antigens expression and type (malignant vs. benign) mammary tumors. Based on the results obtained, it is suggested that each of the studied antigens can be treated as a tumor-associated antigen of canine mammary tumors. However, only the T and Tn antigens seem to be associated with malignant transformation of mammary gland cells and to be of potential value as diagnostic markers.

• Authors: M Nowak, J Madej, P Dziegiel, W Lopuszynski, A Rodo, M Ugorski
• Journal: Veterinary pathology (Vet Pathol) Vol. 46 Issue 2 Pg. 222-6 (Mar 2009) ISSN: 0300-9858 [Print] United States
• PMID: 19261632 (Publication Type: Journal Article)

Chemical References

• Antigens, Tumor-Associated, Carbohydrate
• Biomarkers, Tumor
• CA-19-9 Antigen
• Gangliosides
• sialosyl-Tn antigen
• sialyl Le(a) ganglioside

Topics

• Adenoma (metabolism, veterinary)
• Animals
• Antigens, Tumor-Associated, Carbohydrate (metabolism)
• Biomarkers, Tumor (metabolism)
• CA-19-9 Antigen
• Carcinoma (classification, metabolism, veterinary)
• Dog Diseases (metabolism, pathology)
• Dogs
• Female
• Gangliosides (metabolism)
• Mammary Glands, Animal (cytology, metabolism)
• Mammary Neoplasms, Animal (metabolism, pathology)