Abstract | OBJECTIVES: The beta-2 adrenergic receptor is involved in mediating vasodilatation via neurohumoral and sympathetic nervous system pathways. Alterations in beta-2 adrenergic receptor gene expression ( mRNA transcription) may contribute to the hypertensive phenotype. Human gene expression in clinical phenotypes remains largely unexplored due to ethical constraints involved in obtaining human tissue. We devised a method to obtain normally discarded internal mammary artery tissue from coronary artery bypass graft patients. We then investigated differences in hypertensive and normotensive participants' beta-2 adrenergic receptor gene expression in this tissue. METHODS: We collected arterial tissue samples from 46 coronary artery bypass patients in a surgical setting. Using 41 of the samples, we performed TaqMan real-time polymerase chain reaction (RT-PCR) and used the delta delta cycle threshold (DeltaDeltaCt) relative quantitation method for determination of fold-differences in gene expression between normotensive and hypertensive participants. The beta-2 adrenergic receptor target was normalized to glyceraldehyde- phosphate dehydrogenase. RESULTS: Participants with hypertension had significantly less-expressed beta-2 adrenoceptor gene (2.76-fold, p<.05) compared to normotensive participants. After Bonferroni correction, gene expression did not differ by race, gender, type/dose of beta-blocker prescribed, positive family history of hypertension, or diagnosis of diabetes mellitus type 2. CONCLUSIONS: These data support the possibility of a molecular basis for impaired adrenoceptor-mediated vascular tone in hypertension. Modification and extension of this research is required.
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Authors | Jennifer R Dungan, Yvette P Conley, Taimour Y Langaee, Julie A Johnson, Shawn M Kneipp, Philip J Hess, Carolyn B Yucha |
Journal | Biological research for nursing
(Biol Res Nurs)
Vol. 11
Issue 1
Pg. 17-26
(Jul 2009)
ISSN: 1099-8004 [Print] United States |
PMID | 19254913
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Messenger
- Receptors, Adrenergic, beta-2
- Glyceraldehyde-3-Phosphate Dehydrogenases
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Topics |
- Case-Control Studies
- Clinical Nursing Research
- Coronary Artery Bypass
- Effect Modifier, Epidemiologic
- Female
- Florida
- Gene Expression
(genetics)
- Genetic Testing
- Glyceraldehyde-3-Phosphate Dehydrogenases
(genetics)
- Humans
- Hypertension
(genetics)
- Male
- Mammary Arteries
(cytology, transplantation)
- Middle Aged
- Multivariate Analysis
- Phenotype
- RNA, Messenger
(genetics)
- Receptors, Adrenergic, beta-2
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Statistics, Nonparametric
- Transcription, Genetic
(genetics)
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