Cytotoxicity of cardenolides and cardenolide glycosides from Asclepias curassavica.

Abstract

A new cardenolide, 12beta,14beta-dihydroxy-3beta,19-epoxy-3alpha-methoxy-5alpha-card-20(22)-enolide (6), and a new doubly linked cardenolide glycoside, 12beta-hydroxycalotropin (13), together with eleven known compounds, coroglaucigenin (1), 12beta-hydroxycoroglaucigenin (2), calotropagenin (3), desglucouzarin (4), 6'-O-feruloyl-desglucouzarin (5), calotropin (7), uscharidin (8), asclepin (9), 16alpha-hydroxyasclepin (10), 16alpha-acetoxycalotropin (11), and 16alpha-acetoxyasclepin (12), were isolated from the aerial part of ornamental milkweed, Asclepias curassavica and chemically elucidated through spectral analyses. All the isolates were evaluated for their cytotoxic activity against HepG2 and Raji cell lines. The results showed that asclepin (9) had the strongest cytotoxic activity with an IC(50) value of 0.02 microM against the two cancer cell lines and the new compound 13 had significant cytotoxic activity with IC(50) values of 0.69 and 1.46 microM, respectively.

• Authors: Jun-Zhu Li, Chen Qing, Chang-Xiang Chen, Xiao-Jiang Hao, Hai-Yang Liu
• Journal: Bioorganic & medicinal chemistry letters (Bioorg Med Chem Lett) Vol. 19 Issue 7 Pg. 1956-9 (Apr 01 2009) ISSN: 1464-3405 [Electronic] England
• PMID: 19251412 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 12,14-dihydroxy-3,19-epoxy-3-methoxy-5-card-20(22)-enolide
• 12-hydroxycalotropin
• Cardenolides
• Glycosides

Topics

• Asclepias (chemistry)
• Cardenolides (chemistry, isolation & purification, toxicity)
• Cell Line
• Glycosides (chemistry, isolation & purification, toxicity)
• Humans