Abstract | UNLABELLED:
Prostate cancer is one of the leading causes of death among men in the USA. OBJECTIVE: MATERIALS AND METHODS: RESULTS: Treatment of prostate RWPE-1 cells with PKC-iota silencing RNA ( siRNA) decreased cell viability,cell-cycle accumulation at G2/M phase, and phosphorylation of Cdk7 and Cdk2. In addition, PKC-iota siRNA treatment caused less phosphorylation ofBad at ser-155, ser-136, and greater Bad/Bcl-xL heterodimerization, leading to apoptosis. In DU-145 cells, PKC-iota was anti-apoptotic and was required for cell survival. Treatment with PKC-iota siRNA blocked increase in cell number, and inhibited G1/S transition by accumulation of cells in G0/G1phase. In addition to cell-cycle arrest, both RWPE-1 and DU-145 cells underwent apoptosis due to mitochondrial dysfunction and apoptosis cascades, such as release of cytochrome c,activation of caspase-7, and poly (ADP-ribose)polymerase (PARP) cleavage. CONCLUSION: Our results suggest that PKC-iota is required for cell survival in both transformed non-malignant prostate RWPE-1 cells and androgen-independent malignant prostate DU-145 cells, whereas suppressing PKC-iota lead to apoptosis in DU-145 prostate cells.
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Authors | H Y Win, M Acevedo-Duncan |
Journal | Cell proliferation
(Cell Prolif)
Vol. 42
Issue 2
Pg. 182-94
(Apr 2009)
ISSN: 1365-2184 [Electronic] England |
PMID | 19243387
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- BAD protein, human
- BCL2L1 protein, human
- Isoenzymes
- RNA, Small Interfering
- bcl-Associated Death Protein
- bcl-X Protein
- Poly(ADP-ribose) Polymerases
- Protein Kinase C
- protein kinase C lambda
- Cyclin-Dependent Kinase 2
- Cyclin-Dependent Kinases
- Cyclin-Dependent Kinase-Activating Kinase
- CDK7 protein, human
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Topics |
- Apoptosis
(physiology)
- Cell Count
- Cell Cycle
(physiology)
- Cell Line, Transformed
- Cell Line, Tumor
- Cell Survival
(physiology)
- Cyclin-Dependent Kinase 2
(metabolism)
- Cyclin-Dependent Kinases
(metabolism)
- Humans
- Isoenzymes
(metabolism, physiology)
- Male
- Phosphorylation
- Poly(ADP-ribose) Polymerases
(metabolism)
- Prostate
(enzymology, metabolism, pathology)
- Prostatic Neoplasms
(enzymology, metabolism, pathology)
- Protein Kinase C
(metabolism, physiology)
- RNA, Small Interfering
(genetics)
- bcl-Associated Death Protein
(metabolism)
- bcl-X Protein
(metabolism)
- Cyclin-Dependent Kinase-Activating Kinase
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