Abstract | OBJECTIVE: In critically ill patients, preventing hyperglycemia (HG) with insulin therapy partially prevented organ dysfunction and protected mitochondria. A study in a rabbit model of critical illness indicated that lower blood glucose level, rather than higher insulinemia, is a key factor in such organ protection. In this model, we now investigated the impact of blood glucose lowering vs. hyperinsulinemia (HI) on mitochondria in relation to organ damage. We assessed whether such effects on mitochondria are mediated indirectly via organ perfusion or directly via reducing cellular glucose toxicity. DESIGN: Prospective, randomized laboratory investigation. SETTING: University laboratory. SUBJECTS: Three-month-old male rabbits. INTERVENTIONS: MEASUREMENTS AND MAIN RESULTS: In liver, the reduced damage by glucose lowering was not explained by better perfusion/ oxygen delivery. Abnormal mitochondrial ultrastructure and function was present in the two hyperglycemic groups, most pronounced with concomitant HI. Affected mitochondrial respiratory chain enzyme activities were reduced to 25% to 62% of values in healthy rabbits, in the presence of up to five-fold increased tissue levels of glucose. This was accompanied by elevated levels of dicarbonyls, which may mediate direct toxicity of cellular glucose overload and accelerated glycolysis. The abnormalities were also present in myocardium, although to a lesser extent, and absent in skeletal muscle. CONCLUSIONS: In a rabbit model of critical illness, HG evokes cellular glucose overload in liver and myocardium inducing mitochondrial dysfunction, which explained the HG-induced organ damage. Maintenance of normoglycemia, but not HI, protects against such mitochondrial and organ damage.
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Authors | Ilse Vanhorebeek, Björn Ellger, Rita De Vos, Magaly Boussemaere, Yves Debaveye, Sarah Vander Perre, Naila Rabbani, Paul J Thornalley, Greet Van den Berghe |
Journal | Critical care medicine
(Crit Care Med)
Vol. 37
Issue 4
Pg. 1355-64
(Apr 2009)
ISSN: 1530-0293 [Electronic] United States |
PMID | 19242345
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Blood Glucose
(analysis)
- Burns
(blood, complications, metabolism)
- Critical Illness
- Disease Models, Animal
- Glucose
(toxicity)
- Hyperglycemia
(etiology)
- Hyperinsulinism
(etiology)
- Male
- Mitochondria
- Rabbits
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