Correlation between sPLA2-IIA and phosgene-induced rat acute lung injury.

Abstract

Secreted phospholipase A(2) of group IIA (sPLA(2)-IIA) has been involved in a variety of inflammatory diseases, including acute lung injury. However, the specific role of sPLA(2)-IIA in phosgene-induced acute lung injury remains unidentified. The aim of the present study was to investigate the correlation between sPLA(2)-IIA activity and the severity of phosgene-induced acute lung injury. Adult male rats were randomly exposed to either normal room air (control group) or a concentration of 400 ppm phosgene (phosgene-exposed group) for there are 5 phosgene-exposed groups altogether. For the time points of 1, 3, 6, 12 and 24 h post-exposure, one phosgene-exposed group was sacrificed at each time point. The severity of acute lung injury was assessed by Pa(O2)/F(IO2) ratio, wet-to-dry lung-weight ratio, and bronchoalveolar lavage (BAL) fluid protein concentration. sPLA(2)-IIA activity in BAL fluid markedly increased between 1 h and 12 h after phosgene exposure, and reached its highest level at 6 h. Moreover, the trend of this elevation correlated well with the severity of lung injury. These results indicate that sPLA(2)-IIA probably participates in phosgene-induced acute lung injury.

• Authors: Hong-li Chen, Chun-xu Hai, Xin Liang, Xiao-di Zhang, Riu Liu, Xu-jun Qin
• Journal: Inhalation toxicology (Inhal Toxicol) Vol. 21 Issue 4 Pg. 374-80 (Feb 2009) ISSN: 1091-7691 [Electronic] England
• PMID: 19235614 (Publication Type: Journal Article)

Chemical References

• Chemical Warfare Agents
• RNA, Messenger
• Tumor Necrosis Factor-alpha
• Phosgene
• Interleukin-10
• Group II Phospholipases A2
• Oxygen

Topics

• Animals
• Blood Gas Analysis
• Bronchoalveolar Lavage Fluid
• Capillary Permeability (drug effects)
• Chemical Warfare Agents (toxicity)
• Group II Phospholipases A2 (biosynthesis, genetics)
• Interleukin-10 (biosynthesis)
• Lung (pathology)
• Lung Diseases (chemically induced, enzymology, pathology)
• Male
• Organ Size
• Oxygen (blood)
• Phosgene (toxicity)
• RNA, Messenger (biosynthesis, genetics)
• Rats
• Rats, Sprague-Dawley
• Tumor Necrosis Factor-alpha (biosynthesis)