Abstract | BACKGROUND: Immunization of mice with the G protein of respiratory syncytial virus (RSV) characteristically induces an immune response that is partially protective, but which can prime for pulmonary eosinophilia. We have shown previously that the N191A mutation in a recombinant RSV G protein fragment is associated with reduced pulmonary eosinophilic infiltration when administered with alum subcutaneously in BALB/c mice followed by RSV challenge. We hypothesize that the performance of this " epitope enhanced" recombinant G protein fragment may be further improved by combining with the newly developed adjuvant, Protollin, coupled with intranasal delivery. OBJECTIVES: STUDY DESIGN: RESULTS: CONCLUSIONS: These results support the concept of an RSV vaccine optimized by combined strategies, including epitope enhancement and judicious selection of adjuvants.
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Authors | Yan Huang, Sonya L Cyr, David S Burt, Robert Anderson |
Journal | Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
(J Clin Virol)
Vol. 44
Issue 4
Pg. 287-91
(Apr 2009)
ISSN: 1873-5967 [Electronic] Netherlands |
PMID | 19233722
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Antibodies, Viral
- Drug Combinations
- Immunoglobulin G
- Lipopolysaccharides
- Protollin
- Respiratory Syncytial Virus Vaccines
- Vaccines, Subunit
- Vaccines, Synthetic
- Viral Envelope Proteins
- attachment protein G
- Cysteine Endopeptidases
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Topics |
- Adjuvants, Immunologic
(administration & dosage, pharmacology)
- Administration, Intranasal
- Animals
- Antibodies, Viral
(blood)
- Cysteine Endopeptidases
(administration & dosage, pharmacology)
- Drug Combinations
- Eosinophilia
(prevention & control)
- Female
- Immunoglobulin G
(blood)
- Lipopolysaccharides
(administration & dosage, pharmacology)
- Lung
(virology)
- Mice
- Mice, Inbred BALB C
- Respiratory Syncytial Virus Infections
(prevention & control)
- Respiratory Syncytial Virus Vaccines
(administration & dosage, genetics, immunology)
- Respiratory Syncytial Viruses
(genetics, immunology)
- Vaccines, Subunit
(administration & dosage, genetics, immunology)
- Vaccines, Synthetic
(administration & dosage, genetics, immunology)
- Viral Envelope Proteins
(administration & dosage, genetics, immunology)
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