Evaluation of the genotoxicity of Fusarium mycotoxin moniliformin in human peripheral blood lymphocytes.

Abstract

Mycotoxins are fungal secondary metabolites that can be found in contaminated food and feed. There is some evidence to suggest that certain mycotoxins may be mutagenic. Here, we investigate the genotoxicity of the mycotoxin moniliformin (MON) (3-hydroxycyclobut-3-ene-1,2-dione) in human peripheral blood lymphocytes using chromosomal aberration (CA), sister-chromatid exchange (SCE), and micronucleus (MN) analysis. Lymphocyte cultures were treated for 48 h with six different concentrations of MON between 2.5 and 25 microM. CA, SCE, and MN frequencies were significantly increased in a dose-dependent manner compared with the negative control. The mitotic, replication, and cytokinesis-block proliferation indices were not affected by treatment with MON. The results provide evidence to demonstrate that MON can exert cytogenetic effects in human cells in culture.

• Authors: Mustafa Celik, Serkan Yilmaz, Hüseyin Aksoy, Fatma Unal, Deniz Yüzbaşioğlu, Lale Dönbak
• Journal: Environmental and molecular mutagenesis (Environ Mol Mutagen) Vol. 50 Issue 5 Pg. 431-4 (Jun 2009) ISSN: 1098-2280 [Electronic] United States
• PMID: 19230001 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyclobutanes
• Mycotoxins
• moniliformin

Topics

• Adult
• Cells, Cultured
• Chromosome Aberrations (chemically induced)
• Cyclobutanes (chemistry, toxicity)
• Fusarium (metabolism)
• Humans
• Lymphocytes (drug effects, metabolism)
• Micronuclei, Chromosome-Defective (chemically induced)
• Micronucleus Tests
• Molecular Structure
• Mycotoxins (chemistry, toxicity)
• Sister Chromatid Exchange (drug effects)
• Young Adult