Overexpression of bacterioferritin comigratory protein (Bcp) enhances viability and reduced glutathione level in the fission yeast under stress.

Abstract

The structural gene encoding bacterioferritin comigratory protein (Bcp) was amplified using PCR from the genomic DNA of Schizosaccharomyces pombe, and transferred into the shuttle vector pRS316 to generate the recombinant plasmid pBCPlO. The bcp(+) mRNA level in the pBCPlO-containing yeast cells was significantly higher than that in the control yeast cells, indicating that the cloned gene is functioning. The S. pombe cells harboring the plasmid pBCPIO exhibited higher survival on the solid minimal media with hydrogen peroxide, tert-BOOH or cadmium than the control yeast cells. They also exhibited enhanced cellular viability in the liquid media containing the stressful agents. The increased viabilities of the fission yeast cells harboring the plasmid pBCP10 were also obtained with 0.4% glucose or 0.4% sucrose as a sole carbon source, and nitrogen starvation, compared with those of the control yeast cells. The total glutathione (GSH) content and total GSH/GSSG ratio were significantly higher in the yeast cells harboring the plasmid pBCP10 than in the control yeast cells. In brief, the S. pombe Bcp plays a protective role in the defensive response to oxidative stress possibly via up-regulation of total and reduced glutathione levels.

• Authors: Ga-Young Kang, Eun-Hee Park, Kyunghoon Kim, Chang-Jin Lim
• Journal: Journal of microbiology (Seoul, Korea) (J Microbiol) Vol. 47 Issue 1 Pg. 60-7 (Feb 2009) ISSN: 1976-3794 [Electronic] Korea (South)
• PMID: 19229492 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Fungal
• DNA, Recombinant
• Reactive Oxygen Species
• Schizosaccharomyces pombe Proteins
• Peroxiredoxins
• SPBC1773.02c protein, S pombe
• Glutathione

Topics

• Amino Acid Sequence
• Base Sequence
• Consensus Sequence (genetics)
• DNA, Fungal (isolation & purification)
• DNA, Recombinant (biosynthesis)
• Glutathione (biosynthesis)
• Microbial Viability (genetics)
• Molecular Sequence Data
• Oxidative Stress (genetics)
• Peroxiredoxins (biosynthesis, genetics)
• Reactive Oxygen Species (metabolism)
• Schizosaccharomyces (chemistry, enzymology, genetics)
• Schizosaccharomyces pombe Proteins (biosynthesis, chemistry, genetics)
• Sequence Analysis, DNA
• Up-Regulation