Abstract | BACKGROUND: METHODS: Fifty-two pairs of OSCC cells and normal oral mucosal cells were obtained by brush biopsy from OSCC patients. mRNA was extracted from cell pellets for real-time quantitative reverse transcriptase polymerase chain reaction (RT-qPCR). The over-expression levels of CK 17, CK 19 and CK 20 mRNA in OSCC cells were examined by SYBR green real-time RT-qPCR. RESULTS: Compared to normal mucosal cells, the over-expression of CK 17 mRNA was detectable in 40 OSCC cells (76.9%), that of CK 19 mRNA in 19 (36.5%), while that of CK 20 mRNA was not detectable. Compared with CK 19, the mean value of CK 17 mRNA expression level was significantly higher in all 52 patients (P < 0.02). Moreover, the value of CK 17 was significantly higher in T1 and T2 OSCC patients (P < 0.03, respectively), in patients without metastases of neck lymph nodes (P < 0.04), in stage I and stage II patients (P < 0.03 and P < 0.05, respectively) and in well differentiated OSCC patients (P < 0.05). CONCLUSION: Brush biopsy properly serves for detection of CK mRNA using real-time RT-qPCR. This preliminary study demonstrates the CK 17 possibility for application; however, pivotal studies are encouraged to confirm CK 17 as a diagnostic marker of OSCC in a brush biopsy test.
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Authors | T Toyoshima, F Koch, P Kaemmerer, E Vairaktaris, B Al-Nawas, W Wagner |
Journal | Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
(J Oral Pathol Med)
Vol. 38
Issue 6
Pg. 530-4
(Jul 2009)
ISSN: 1600-0714 [Electronic] Denmark |
PMID | 19222712
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Keratin-17
- Keratin-19
- Keratin-20
- RNA, Messenger
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Topics |
- Biomarkers, Tumor
(analysis)
- Carcinoma, Squamous Cell
(diagnosis, pathology, secondary)
- Cell Line, Tumor
- Cytodiagnosis
(instrumentation, methods)
- Gene Expression Regulation
- Humans
- Keratin-17
(analysis)
- Keratin-19
(analysis)
- Keratin-20
(analysis)
- Lymphatic Metastasis
(pathology)
- Mouth Mucosa
(pathology)
- Mouth Neoplasms
(diagnosis, pathology)
- Neoplasm Staging
- RNA, Messenger
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
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