Local application of hepatocyte growth factor using gelatin hydrogels attenuates noise-induced hearing loss in guinea pigs.

Abstract

CONCLUSION:

Local application of hepatocyte growth factor using biodegradable gelatin hydrogels attenuates noise-induced hearing loss in guinea pigs.

OBJECTIVES:

To develop an inner ear drug delivery system using gelatin hydrogels that is capable of a sustained delivery of growth factors to the cochlea. We examined the efficacy of the local application of gelatin hydrogels containing hepatocyte growth factor (HGF) in protecting cochlear hair cells from noise-induced damage.

MATERIALS AND METHODS:

A piece of gelatin hydrogel previously immersed in either HGF or saline was placed on the round window membrane of a guinea pig 1 h after noise exposure (4 kHz octave band noise at 120 dB sound pressure level for 3 h). Auditory function was monitored using auditory brainstem responses (ABRs), and the loss of hair cells was evaluated quantitatively.

RESULTS:

Local HGF treatment significantly reduced the noise exposure-caused ABR threshold shifts and the loss of outer hair cells in the basal portion of the cochleae.

• Authors: Takatoshi Inaoka, Takayuki Nakagawa, Yayoi S Kikkawa, Yasuhiko Tabata, Kazuya Ono, Mitsuhiro Yoshida, Hirohito Tsubouchi, Akio Ido, Juichi Ito
• Journal: Acta oto-laryngologica (Acta Otolaryngol) Vol. 129 Issue 4 Pg. 453-7 (Apr 2009) ISSN: 1651-2251 [Electronic] England
• PMID: 19214837 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hydrogels
• Hepatocyte Growth Factor

Topics

• Animals
• Audiometry
• Evoked Potentials, Auditory, Brain Stem
• Guinea Pigs
• Hair Cells, Auditory (pathology)
• Hearing Loss, Noise-Induced (drug therapy, pathology)
• Hepatocyte Growth Factor (administration & dosage)
• Hydrogels (administration & dosage)
• Male