Abstract | PURPOSE: We evaluated the usefulness of small animal brain positron emission tomography (PET) imaging with the amyloid-beta (A beta) probe 2-(1-{6-[(2-[(18)F]fluoroethyl)(methyl)amino]-2-naphthyl}ethylidene) malonitrile ([( 18)F]FDDNP) and with 2-deoxy-2-[F-18]fluoro- D-glucose (FDG) for detection and quantification of pathological changes occurring in a transgenic mouse model of Alzheimer's disease (Tg2576 mice). PROCEDURES: [( 18)F]FDDNP (n = 6) and FDG-PET scans (n = 3) were recorded in Tg2576 mice (age 13-15 months) and age-matched wild-type litter mates. Brain volumes of interest were defined by co-registration of PET images with a 3D MOBY digital mouse phantom. Regional [( 18)F]FDDNP retention in mouse brain was quantified in terms of the relative distribution volume (DVR) using Logan's graphical analysis with cerebellum as a reference region. RESULTS: Except for a lower maximum brain uptake of radioactivity in transgenic animals, the regional brain kinetics as well as DVR values of [( 18)F]FDDNP appeared to be similar in both groups of animals. Also for FDG, regional radioactivity retention was almost identical in the brains of transgenic and control animals. CONCLUSIONS: We could not detect regionally increased [( 18)F]FDDNP binding and regionally decreased FDG binding in the brains of Tg2576 transgenic versus wild-type mice. However, small group differences in signal might have been masked by inter-animal variability. In addition, technical limitations of the applied method (partial volume effect, spatial resolution) for measurements in such small organs as mouse brain have to be taken into consideration.
|
Authors | Claudia Kuntner, Adam L Kesner, Martin Bauer, Robert Kremslehner, Thomas Wanek, Markus Mandler, Rudolf Karch, Johann Stanek, Tanja Wolf, Markus Müller, Oliver Langer |
Journal | Molecular imaging and biology
(Mol Imaging Biol)
2009 Jul-Aug
Vol. 11
Issue 4
Pg. 236-40
ISSN: 1860-2002 [Electronic] United States |
PMID | 19214638
(Publication Type: Journal Article)
|
Chemical References |
- 2-(1-(6-((2-fluoroethyl)(methyl)amino)-2-naphthyl)ethylidene)malononitrile
- Amyloid beta-Peptides
- Nitriles
- Radiopharmaceuticals
- Fluorodeoxyglucose F18
|
Topics |
- Alzheimer Disease
(diagnostic imaging, pathology)
- Amyloid beta-Peptides
(metabolism)
- Animals
- Brain
(diagnostic imaging, pathology)
- Brain Chemistry
- Disease Models, Animal
- Fluorodeoxyglucose F18
(pharmacokinetics)
- Imaging, Three-Dimensional
(methods)
- Mice
- Mice, Transgenic
- Nitriles
(pharmacokinetics)
- Phantoms, Imaging
- Positron-Emission Tomography
(methods)
- Radiopharmaceuticals
(pharmacokinetics)
- Statistics, Nonparametric
|