Forensic toxicological examination was performed on a person who was suspected of
poisoning by Azomite
emulsion (an
acaricide),
Roundup (a
herbicide) and/or unidentified
agricultural chemical and died after 4 days. In Japan, production of Azomite
emulsion has been stopped since 1973. The unidentified
agricultural chemical was identified as an
agricultural chemical containing
malathion, an organo phosphorous compound, by analyzing with gas chromatograph-mass spectrometer (GC-MS). When the person's serum was qualitatively analyzed with GC-MS,
aramite and
azoxybenzene, effective components of Azomite
emulsion, were detected but not
glyphosate, effective component of
Roundup, and
malathion. And so, quantitative analysis of
aramite and
azoxybenzene were performed with mass fragmentography.
Aramite and
azoxybenzene concentrations in the serum obtained at about 9 hours after he took Azomite
emulsion were 5.20 micrograms/ml and 112 micrograms/ml, respectively. Only trace amounts of them were, however, detected in the serum obtained at about 3.5 days after that. Both
aramite and
azoxybenzene existed significantly in liver of the
corpse, and their concentrations were 12.0 micrograms/g and 254 micrograms/g, respectively. Relatively large amounts of them were also deposited in the kidney. Severe liver and kidney disorders shown in this case were considered to be mainly induced by deposits of
aramite and
azoxybenzene in the liver and kidney. Also in the future,
poisoning by
agricultural chemicals that productions have been stopped like Azomite
emulsion may happen. Therefore, it may be necessary for forensic toxicologists and medical doctors in emergency hospitals to try to be able to cope promptly with such
poisoning cases.