Abstract | BACKGROUND: MATERIALS AND METHODS: Expression of AMPs was quantified by real-time PCR in biopsies obtained from healthy individuals and patients with infectious and non-infectious gastritis as well as in AGS gastric epithelial cells infected with H. pylori. Distribution of hBD-4 in the gastric mucosa was characterized by in-situ hybridisation and immunohistochemistry. The role of Toll-like receptors (TLRs) 2 and 4 and associated signalling pathways was addressed. RESULTS: hBD-4 was expressed at low levels in gastric epithelial cells and was significantly upregulated in infectious and non-infectious gastritis. Standard eradication but not acid suppression therapy significantly decreased hBD-4 expression. Cytotoxin associated gene (cag)A positive H. pylori significantly increased the expression of hBD-4 whereas cagA negative organisms, non-viable bacteria or culture supernatants had no significant effect. Overexpression and downregulation of TLRs was not associated with an altered hBD-4 expression. However, blocking experiments revealed an essential role for the p38 mitogen-activated protein kinase. RNase7 was inconsistently expressed in biopsies and not significantly upregulated by H. pylori. CONCLUSIONS: hBD-4 may play a significant role in H. pylori associated gastritis. Inconsistent expression of RNase 7 does not support a pivotal role for this peptide in response to infection with H. pylori.
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Authors | J-M Otte, H M Neumann, S Brand, H Schrader, W E Schmidt, F Schmitz |
Journal | European journal of clinical investigation
(Eur J Clin Invest)
Vol. 39
Issue 2
Pg. 126-38
(Feb 2009)
ISSN: 1365-2362 [Electronic] England |
PMID | 19200166
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antimicrobial Cationic Peptides
- Cathelicidins
- Toll-Like Receptor 2
- Toll-Like Receptor 4
- beta-Defensins
- Ribonucleases
- Ribonuclease 7
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Antimicrobial Cationic Peptides
(metabolism)
- Case-Control Studies
- Cathelicidins
- Female
- Gastric Mucosa
(metabolism, microbiology)
- Gastritis
(metabolism, microbiology)
- Gene Expression
- Helicobacter Infections
(metabolism, microbiology)
- Helicobacter pylori
- Humans
- Male
- Middle Aged
- Ribonucleases
(metabolism)
- Toll-Like Receptor 2
(metabolism)
- Toll-Like Receptor 4
(metabolism)
- Young Adult
- beta-Defensins
(metabolism)
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