Abstract | BACKGROUND:
Insulinoma is an autonomous insulin-secreting islet cell neoplasm that is rarely diagnosed in cats. The clinical and pathological aspects of feline insulinoma have been described previously, but the molecular characteristics of these tumors have not been investigated. OBJECTIVES: The study objectives were to characterize peptide hormone production and determine expression of selected genes involved in glucose metabolism and insulin secretion in a feline insulinoma. METHODS: Immunohistochemistry and RT-PCR were used to examine hormone and gene expression, respectively, by insulinoma cells. RESULTS: CONCLUSIONS AND CLINICAL IMPORTANCE: The feline insulinoma produced several peptide hormones and expressed genes consistent with a beta-cell phenotype. The pattern of hexokinase gene expression in tumor cells differed from that of normal pancreas. These findings suggest insulinoma cells may have an increased sensitivity to glucose that could contribute to the abnormal insulin secretory response observed at low serum glucose concentrations.
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Authors | T C Jackson, B Debey, S Lindbloom-Hawley, B T Jones, T Schermerhorn |
Journal | Journal of veterinary internal medicine
(J Vet Intern Med)
2009 Mar-Apr
Vol. 23
Issue 2
Pg. 383-7
ISSN: 0891-6640 [Print] United States |
PMID | 19192149
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Neoplasm
- Glucose Transporter Type 2
- Insulin
- Hexokinase
- Glucokinase
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Topics |
- Animals
- Cat Diseases
(genetics, metabolism)
- Cats
- DNA, Neoplasm
(genetics, metabolism)
- Gene Expression
- Glucokinase
(biosynthesis, genetics)
- Glucose Transporter Type 2
(biosynthesis, genetics)
- Hexokinase
(biosynthesis, genetics)
- Immunohistochemistry
(veterinary)
- Insulin
(biosynthesis, genetics)
- Insulin-Secreting Cells
(metabolism)
- Insulinoma
(genetics, metabolism, veterinary)
- Pancreatic Neoplasms
(genetics, metabolism, veterinary)
- Polymerase Chain Reaction
(veterinary)
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