Determination of the phenoxyacid herbicides MCPA, mecoprop and 2,4-D in kidney tissue using liquid chromatography with electrospray tandem mass spectrometry.

Abstract

An analytical method was developed to determine the phenoxyacid herbicides 2,4-D, MCPA and mecoprop in kidney tissue from animals where poisoning is suspected. Samples were Soxhlet extracted using diethyl ether and the extracts cleaned-up using anion exchange solid phase extraction cartridges. Analysis was performed using liquid chromatography with negative-ion electrospray tandem mass spectrometry (LC-MS/MS). The method was evaluated by analysing control kidney samples fortified at 1 and 5 mg/kg. Mean recoveries ranged from 82 to 93% with relative standard deviations from 3.2 to 19%. The limit of detection was estimated to be 0.02 mg/kg.

• Authors: Andrew J A Charlton, Vicki Stuckey, Mark D Sykes
• Journal: Bulletin of environmental contamination and toxicology (Bull Environ Contam Toxicol) Vol. 82 Issue 6 Pg. 711-5 (Jun 2009) ISSN: 1432-0800 [Electronic] United States
• PMID: 19183823 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 2,4-Dichlorophenoxyacetic Acid
• mecoprop
• 2-Methyl-4-chlorophenoxyacetic Acid

Topics

• 2,4-Dichlorophenoxyacetic Acid (analysis, isolation & purification, poisoning)
• 2-Methyl-4-chlorophenoxyacetic Acid (analogs & derivatives, analysis, isolation & purification, poisoning)
• Animals
• Chromatography, Liquid (methods)
• Kidney (chemistry)
• Solid Phase Extraction (methods)
• Spectrometry, Mass, Electrospray Ionization (methods)
• Swine
• Tandem Mass Spectrometry